A simple and rapid molecular method for Leptospira species identification |
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| Authors: | Ahmed Ahmed Richard M. Anthony Rudy A. Hartskeerl |
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| Affiliation: | 1. Centre of Medical Laboratory Technology, Faculty Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia;2. Department of Parasitology, Faculty of Medicine and Health Sciences, Sana''a University, Sana''a, Yemen;3. Endemic and Tropical Diseases Unit, Medical Research Center, Jazan University, Jazan, Saudi Arabia;4. Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Sungai Long Campus, Selangor, Malaysia;5. Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia |
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| Abstract: | Serological and DNA-based classification systems only have little correlation. Currently serological and molecular methods for characterizing Leptospira are complex and costly restricting their world-wide distribution and use. Ligation mediated amplification combined with microarray analysis avoids many of these drawbacks. We demonstrated that this approach used in the Check-Points (CP) assay can successfully applied for the generic detection of Leptospira and can discriminate between saprophytic, intermediate and pathogenic species. In addition, the CP assay could unambiguously detect strains of seven pathogenic species and revealed discrepancies in previous speciation and culture collections. The method provides a valuable tool adding to the molecular study of leptospires and their local and global distribution. |
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