Molecular detection of Cryptosporidium cuniculus in rabbits in Australia |
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| Authors: | Matthew J Nolan Aaron R Jex Shane R Haydon Melita A Stevens Robin B Gasser |
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| Institution: | 1. Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia;2. Melbourne Water Corporation, Victoria, Australia;1. Heilongjiang Key Laboratory for Zoonosis, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;2. Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China;3. Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA;1. Parasitology & Entomology Dept., Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran;2. Medical Biotechnology Dept., Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran;1. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China;2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, People’s Republic of China;1. Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;2. Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;3. Department of Virology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;4. Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;5. Department of Animal Genetics, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;1. Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia;2. Henan Agricultural University, Henan, China;3. WaterNSW, Sydney, Australia |
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| Abstract: | In the United Kingdom, rabbits have been reported to harbour genotypes of Cryptosporidium (now recognized as C. cuniculus) identical to those from human patients exhibiting symptoms of cryptosporidiosis. The high density of rabbits in many regions of Australia, including both rural and urban as well as natural water catchments areas, and the absence of any information on Cryptosporidium from lagomorphs in this country stimulated the present study. We undertook an epidemiological study that genetically characterized Cryptosporidium from rabbits from four locations in Victoria by PCR-coupled sequencing and phylogenetic analysis of sequence data for loci within the small subunit of nuclear ribosomal RNA (SSU; for specific identification) and the 60 kDa glycoprotein gene (gp60; for genotypic/subgenotypic identification). Cryptosporidium was detected in 12 (6.8%) of 176 individual faecal samples. For SSU, all 12 sequences were identical to each other and to that of C. cuniculus. For pgp60, all corresponding sequences matched the known genotype Vb, and were classified as subgenotype VbA23R3 (n = 11) and VbA26R4 (n = 1), which are both new records. Present evidence indicates that genotype Vb is limited to rabbits; however, it would be premature to conclude that this genotype is not zoonotic. Future studies should focus on the zoonotic potential of C. cuniculus from rabbits and a wide range of yet unstudied animals. (Nucleotide sequences reported in this paper are available in the GenBank database under accession nos. HM852431–HM852433). |
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