| 基于ITS序列的紫芝分子鉴定 |
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| 引用本文： | 苏春丽,唐传红,张劲松. 基于ITS序列的紫芝分子鉴定[J]. 成都医学院学报, 2014, 9(2): 121-123 |
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| 作者姓名： | 苏春丽 唐传红 张劲松 |
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| 作者单位： | 苏春丽 (成都医学院公共卫生系,成都610500上海市农业科学院食用菌研究所,上海201403)； 唐传红 (上海市农业科学院食用菌研究所,上海,201403)； 张劲松 (上海市农业科学院食用菌研究所,上海,201403)； |
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| 基金项目： | 上海市农委重点攻关项目(项目编号：农科攻字2002-1-4-3)成都医学院学科建设项目(项目编号：CYXK2012010) |
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| 摘 要： | 目的 建立基于ITS序列的紫芝分子鉴定方法.方法 分析20株灵芝属菌株ITS序列,设计紫芝的特异性引物.结果 用此引物对20株灵芝属菌株的DNA进行扩增,只有2株紫芝菌株有目的扩增条带.结论 设计的特异性引物有很高的专属性,能高度特异地鉴定紫芝菌株.
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| 关 键 词： | 紫芝 ITS序列 分子鉴定 |
Molecular Detection of Ganoderma sinense Based on Nuclear Ribosomal DNA ITS Sequence |
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| SU Chun-li,TANG Chuan-hong,ZHANG Jing-song. Molecular Detection of Ganoderma sinense Based on Nuclear Ribosomal DNA ITS Sequence[J]. Journal of Chengdu Medical College, 2014, 9(2): 121-123 |
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| Authors: | SU Chun-li TANG Chuan-hong ZHANG Jing-song |
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| Affiliation: | 1. Department oF Public Health, Chengdu Medical College, Chengdu 610500, China; 2. Institute oF Edible Fungi, Shanghai Academy oF Agricultural Sciences, Shanghai 201403, China) |
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| Abstract: | Objective To build a molecular detection method to identify Ganoderma sinense isolates. Methods Based on phylogenetic analysis of rDNA ITS sequences, A pair of specific primers were designed for identifying the Ganoderrna sinense by PCR. Results 20 isolates of Ganoderma were amplified by the primer pairs, only two Ganoderma sinense isolates had PCR amplification products. Conclusion It is demonstrated that the primer pairs have a high specificity, and can be used to specifically identify Ganoderma sinense isolates. |
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| Keywords: | Ganoderma sinense ITS Sequences Molecular Detection |
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