重组质粒pCKM—mPTH对甲状旁腺功能低下症动物模型血钙的影响

目的研究重组pCKM—mPTH质粒在SD大鼠甲状旁腺功能低下症模型治疗中对血钙浓度的影响。方法将60只SD大鼠制作甲状旁腺功能低下症模型后，随机分为3组，分别肌注pcDNA3．1（＋）空质粒、mPTH质粒和pCKM—mPTH质粒，不同时间用放免法测定血清甲状旁腺素（PTH）浓度，采用MTB比色法检测血清钙浓度。结果动物模型在注射mPTH和pCKM～mPTH质粒后，其血清PTH和钙浓度均出现显著升高，之后出现回落，但pCKM—mPTH质粒组回落较缓慢，其作用维持近30天。结论质粒pCKM—mPTH在SD大鼠体内合成的甲状旁腺素能有效的调节血清钙的浓度，且转染效率和维持时间优于不含pCKM启动子的mPTH质粒。

Influence of recombinant plasmid pCKM-mPTH to the serum calcium concentration of the animal models of hypoparathyroidism

Objectivs To study the influence of recombinant plasmid pCKM-mPTH to the serum calcium concentration of the SD rats of hypoparathyroidism model. Methods 60 SD rats of hypoparathyroidism models were randomly divided into three groups, which were injected intramuscular pcDNA3.1 ( + ) empty vector, mPTH plasmids and pCKM-mPTH plasmid via muscle. At different times, rats were measured serum parathyroid hormone ( PTH ) concentration using radioimmunoassay, and serum calcium concentration using MTB colorimetric assay.Results After being injected with mPTH and pCKM-mPTH plasmid, serum PTH and calcium appeared significantly increased and fell later, but that of pCKM-mPTH plasmid groups fell more slowly, which continued nearly 30 days. Conclusion Recombinant plasmid pCKM-mPTH can effectively regulate the concentration of serum calcium after transfecting SD rats of hypoparathyroidism model. Furthermore, its transfection efficiency and the maintenance of time are superior to non-pCKM promoter mPTH plasmid.