| 紫背天葵中矢车菊素3-O-葡萄糖苷的鉴别及含量测定 |
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| 引用本文： | 谭雄斯,唐铁鑫,周本宏. 紫背天葵中矢车菊素3-O-葡萄糖苷的鉴别及含量测定[J]. 国际医药卫生导报, 2011, 17(5): 600-603. DOI: 10.3760/cma.j.issn.1007-1245.2011.05.034 |
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| 作者姓名： | 谭雄斯 唐铁鑫 周本宏 |
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| 作者单位： | 谭雄斯,TAN Xiong-si(430071,武汉大学药学院;526020,肇庆医学高等专科学校);唐铁鑫,TANG Tie-xin(肇庆医学高等专科学校,526020);周本宏,ZHOU Ben-hong(武汉大学药学院,430071) |
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| 摘 要： | 目的建立紫背天葵中矢车菊素3-O-葡萄糖苷的鉴别及含量测定方法。方法应用薄层色谱法建立鉴别方法，虚用反相高效液相色谱法建立含量测定方法。结果矢车菊素3-O-葡萄糖苷的薄层鉴别方法专属、可靠；液相含量测定方法中，矢车菊素3-O-葡萄糖苷在36～360ng范同内与峰面积的线性关系良好（r=0．9997，n=6），加样回收率98．7％，相对标准偏差值（RSD）=2．02％。结论本研究的方法可用于紫背天葵中矢车菊素3-O-葡萄糖苷的定性定量。
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| 关 键 词： | 紫背天葵 矢车菊素3-O-葡萄糖苷 TLC RP—HPLC 鉴别 含量测定 |
Identification and content determination of cyanidin 3-O-glucoside in Begonia fimbristipula hance |
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| TAN Xiong-si,TANG Tie-xin,ZHOU Ben-hong. Identification and content determination of cyanidin 3-O-glucoside in Begonia fimbristipula hance[J]. International Medicine & Health Guidance News, 2011, 17(5): 600-603. DOI: 10.3760/cma.j.issn.1007-1245.2011.05.034 |
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| Authors: | TAN Xiong-si TANG Tie-xin ZHOU Ben-hong |
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| Affiliation: | .(Zhaoqing Medical College, Zhaoqing 526020, China) |
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| Abstract: | Objective To establish qualitative and quantitative analysis methods of cyanidin 3-O-glucoside in Begonia fimbristipla hance. Methods The qualitative method was established with TLC and the quantitive method was established with RP-HPLC. Results The identification method with TLC for cyanndin 3-O-glucoside was specific and reliable. In the assay, the linearity of cyanidin 3-O-glucside ranged from 36 ng to 360 ng (r= 0.999 7, n = 6), and the recovery was 98.7%, with RSD of 2.02%.Conclusions Our detection method is suitable for qualitative and quantitive analysis of cyanidin3-O-glucoside in Begonia fimbristipula hance. |
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| Keywords: | TLC RP-HPLC |
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