粉防己碱对实验性胸膜炎的防治作用的研究

角叉菜胶（Ｃａｒｒａｇｅｅｎｉｎ，Ｃａｒ）致大鼠胸膜炎后８ｈ，胸膜腔渗出液中中性白细胞游离钙浓度（Ｎｅｕ［Ｃａ＾２＾＋］ｉ）升高，中性白细胞钙调素活性（Ｎｅｕ－ＣａＭＡ）增强，粉防己碱（Ｔｅｔｒａｎｄｒｉｎｅ，Ｔｅｔ）（１０－８０ｍｇ．ｋｇ＾－＾１）于致炎前３０ｍｉｎ和致炎后４ｈ给大鼠灌胃可明显减少炎症胸膜腔渗出液量，蛋白渗出量和白细胞游出数，同时能降低Ｎｅｕ［Ｃａ＾２＾＋］ｉ和Ｎｅｕ－ＣａＭＡ；

Studies of prophylactic and therapeutic effects of tetrandrine on experimental pleuritis in rats

There was an increase of cytosolic free calcium concentration [Neu-Ca(2+)]i and calmodulin activity(Neu-CaMA)of the neutrophils in the pleural exudate at 8 h after carrageenin-induced pleuritis in rats. When tetrandrine(Tet) was intragastrically administered in the dosages of 10, 20, 40 and 80 mg.kg(-1) to rats at 30 min prior to and 4 h after the infliction of pleuritis, the amount, protein content and neutrophil count of the pleural exudate could significantly reduced,[Neu-Ca(2+)]i was decreased and Neu-CaMA inhibited. In addition, it was found that calcium was able to reverse the inhibition of tetrandrine on Neu-CaMA in vitro.On the basis of these findings, it is believed that the excellent anti-inflammatory effect of Tet may be related to its antagonism to calcium-calmondulin system and the inhibition of Tet on Neu-CaMA is dependent upon the decrease of [Neu-Ca(2+)]i level.