淫羊藿苷磁性纳米脂质体的制备及体外释放情况分析

目的: 优选淫羊藿苷磁性纳米脂质体的处方工艺并考察其体外释放情况,为改善淫羊藿苷的生物利用度提供参考. 方法: 采用超声薄膜分散法制备淫羊藿苷磁性纳米脂质体,以淫羊藿苷包封率为指标,在单因素试验基础上,通过正交试验考察油酸改性磁粉、泊洛沙姆188(F-68)、聚乙二醇-2000(PEG-2000)投料量及水化介质对处方工艺的影响.利用HPLC考察淫羊藿苷磁性纳米脂质体在含30%甲醇的磷酸盐缓冲液(PBS)中的体外释放率,流动相乙腈-水(30:70),检测波长270 nm. 结果: 最优处方为淫羊藿苷-磁粉(2:1),F-68 200 mg,PEG-2000 40 mg,水化介质为PBS(pH 7.4),大豆卵磷脂500 mg,胆固醇150 mg.淫羊藿苷磁性纳米脂质体包封率(96.57±0.42)%,72 h累积释放率96.51%;淫羊藿苷溶液16 h释放99.68%. 结论: 通过超声薄膜分散法制备的淫羊藿苷磁性纳米脂质体具有较高包封率和良好的缓释效果.

Preparation and in vitro Release of Icariin Magnetic Nano-liposomes

Objective: To optimize formulation of icariin magnetic nano-liposomes and investigate their in vitro release. Method: Ultrasonic film dispersion method was used to prepare icariin magnetic nano-liposomes,with encapsulation efficiency of icariin as index,based on single factor tests,orthogonal design was adopted to optimize preparation procedure by taking hydration medium and amounts of poloxamer 188(F-68),PEG-2000,oleic acid-modified magnetic powder.HPLC was employed to investigate in vitro release of icariin magnetic nano-liposomes in phosphate buffer solution(PBS) containing 30% methanol,mobile phase consisted of acetonitrile-water(30:70) and detection wavelength was set at 270 nm. Result: Optimum formulation was as follows:icariin-magnetic powder(2:1),F-68 of 200 mg,PEG-2000 of 60 mg,lecithin of 500 mg,cholesterol of 150 mg,PBS(pH 7.4) as hydration medium.Entrapment efficiency of icariin magnetic nano-liposomes was (96.57±0.42)%;cumulative release rate of icariin solution was near 99.68% in 16 h,but it was over 96.51% in 72 h for icariin magnetic nano-liposomes. Conclusion: Icariin magnetic nano-liposomes have high encapsulation efficiency and a good sustained release by ultrasonic film dispersion method.