蓝氧片对邻苯二甲酸(2-乙基己基)酯致小鼠睾丸超微形态结构损害的保护作用

目的 观察塑化剂邻苯二甲酸(2-乙基己基)酯(DEHP)对小鼠睾丸超微形态结构的影响，并研究蓝氧片(专利药用组合Y523)对小鼠睾丸生精细胞和支持细胞的保护和修复作用及其机制。方法 55只成年♂小鼠随机分为DEHP组、Y523组和对照组。DEHP组以大豆油为溶剂，剂量分别为：0，10，20，50，和100 mg·kg^-1·d^-1，连续30 d灌胃染毒；Y523组以等量大豆油混合1 000 mg蓝氧片原料粉剂连续30 d灌胃给药后重复DEHP组的灌胃染毒过程；对照组以标准饲料同期喂养。采用透射电镜观察小鼠睾丸超微结构的改变。结果 与对照组相比，随着DEHP灌胃染毒剂量的递增，DEHP组小鼠睾丸生精细胞出现胞膜塌陷，核质疏松，小细胞器消失，空泡形成，甚至整个生精细胞层消失；支持细胞胞膜皱缩，核质浓缩，胞质内空泡形成和凋亡小体样结构。与DEHP组相比，蓝氧片组小鼠睾丸生精细胞和支持细胞的超微形态结构改变与对应剂量DEHP灌胃染毒的DEHP组小鼠基本类似，但胞质内线粒体、溶酶体等小细胞器的数量明显增多。结论 DEHP灌胃染毒可致小鼠睾丸生精细胞空泡样变性退化，细胞胀亡和凋亡。蓝氧片对DEHP所致的睾丸损害有一定的保护作用，其机制可能与小鼠睾丸支持细胞内线粒体和溶酶体等细胞器数量的增加有关。

Protective Effect of Blegent on DEHP Damaged Testis in Rats

OBJECTIVE To investigate the damage of di-(2-ethylhexyl) phthalate (DEHP) on the morphological ultrastructure of mouse testis and the protective effects of Blegent(compound Y523) on mouse spermatogonia and sertoli cells. METHODS Fifty-five male mice were randomly divided into three groups: DEHP, Y523 and control group. DEHP dissolved in soya oil was given at the dose of 0, 10, 20, 50 and 100 mg·kg^-1·d^-1 for 30 d by intragastric administration consecutively in DEHP group. Blegent at the dose of 1 000 mg was mixed with same account of soya oil and intragastric administrated consecutively for 30 d in Y523 group before DEHP intragastric administration repeated as that in DEHP group. The mice in control group were given a standard diet. Transmission electron microscopy was used to observe changes of testicular ultrastructure. RESULTS Compared with the control group, with increased dose of DEHP administrated in DEHP group, the spematogenic cell often showed collapsed cell membrane and loosened nucleoplasm. Few organelles and obvious vacuoles formation were visible in the cytoplasm under the electron microscopy. Seminiferous tubule cells decreased in number and even vanished. Collapsed cell membrane, concentrated nucleoplasm and apoptosis-like structure were also detected in the cytoplasm of sertoli cell. Compared with the DEHP group, the structural and morphological change of spematogenic and sertoli cell in Y523 group approximated to that in the DEHP group corresponded to increased dose of DEHP intragastric administrated but the increased count of organelle such as mitochodria and lysosome in the cytoplasm of sertoli cell were observed. CONCLUSION Intragastric administration of DEHP could lead to the ultrastructure changes of sertoli cell with oncosis or apoptosis and obvious vacuoles formation, decreased count of organelles in cytoplasm of spermatogonium. Blegent may protect and repair the damaged testis of mouse by mechanism of increasing the amount of mitochodria and lysosome in cytoplasm of sertoli cells.