| 褪黑素对人胃癌细胞SGC-7901黏附和迁移的影响 |
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| 引用本文： | 陈宜刚,许 蕊,杨 玲,崔 洁,杜 军. 褪黑素对人胃癌细胞SGC-7901黏附和迁移的影响[J]. 南京医科大学学报(自然科学版), 2014, 0(12): 1644-1648 |
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| 作者姓名： | 陈宜刚 许 蕊 杨 玲 崔 洁 杜 军 |
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| 作者单位： | 泰州职业技术学院医学技术学院,江苏 泰州 225300;南京医科大学生理学系,江苏 南京 210029;苏州大学附属第三医院心脏科,江苏 常州 213003;南京医科大学生理学系,江苏 南京 210029;南京医科大学生理学系,江苏 南京 210029 |
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| 基金项目： | 国家自然科学基金青年基金资助项目(81201614);江苏省六大人才高峰 (JY-020) |
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| 摘 要： | 目的 :研究褪黑素对人胃癌细胞SGC-7901黏附和迁移能力的影响及探索可能的分子机制。方法 :用不同浓度的褪黑素孵育人胃癌细胞SGC-7901 24 h,通过免疫印迹方法检测F-actin和G-actin蛋白表达水平,通过免疫荧光法检测细胞骨架结构的改变,通过细胞黏附实验观察胃癌细胞对血管内皮细胞黏附能力的变化,通过划痕实验检测褪黑素对细胞迁移能力的影响。结果:褪黑素使人胃癌细胞SGC-7901 F-actin量明显降低,同时细胞骨架结构发生重组,应力纤维出现断裂;褪黑素可抑制胃癌细胞的迁移及对血管内皮细胞的黏附能力。结论:褪黑素可能通过破坏细胞内应力纤维结构的稳定,最终导致了胃癌细胞的黏附和迁移能力的降低。
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| 关 键 词： | SGC-7901人胃癌细胞 细胞骨架 褪黑素 |
| 收稿时间： | 2014-06-19 |
Melatonin inhibits adhesion and migration of SGC-7901 gastric cancer cells |
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| Chen Yigang,Xu Rui,Yang Ling,Cui Jie and Du Jun. Melatonin inhibits adhesion and migration of SGC-7901 gastric cancer cells[J]. Acta Universitatis Medicinalis Nanjing, 2014, 0(12): 1644-1648 |
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| Authors: | Chen Yigang Xu Rui Yang Ling Cui Jie Du Jun |
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| Affiliation: | College of Medical Technology,Taizhou Polytechnic College,Taizhou 225300;Department of Physiology,NJMU,Nanjing 210029;Department of Cardiology,the Third Affiliated Hospital of Soochow University,Changzhou 213003,China;Department of Physiology,NJMU,Nanjing 210029;Department of Physiology,NJMU,Nanjing 210029 |
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| Abstract: | Objective:To investigate the effects of melatonin on adhesion and migration abilities of human SGC-7901 gastric cancer cells and its role in reorganization of actin cytoskeleton. Methods:SGC-7901 cells were treated with different concentrations of melatonin for 24h. The contents of G-actin(in cytosolic fraction)and F-actin(in cytoskeletal fraction)in cells were measured by Western blot. Cellular F-actin stained with FITC-phalloidin was examined by fluorescence microscopy. Adhesive assay was used to detect the adhesive ability of SGC-7901 cells to HUVECs. Migration rate was measured by wound healing assay. Results:After treated with melatonin for 24h,the celluar F-actin was depolymerized and actin cytoskeleton was rearranged. Consistently,the abilities of adhesion and migration in SGC-7901 cells were significantly reduced compared to the control cells. Conclusion:Melatonin inhibits the abilities of adhesion and migration in SGC-7901 cells,which may be via reorganization of cellular actin cytoskeleton. |
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| Keywords: | SGC-7901 gastric cancer cells cytoskeleton melatonin |
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