CYP2E1和CYP1A1及白细胞介素-4基因多态性与三氯乙烯药疹样皮炎的关系

目的 研究代谢酶基因CYP2E1和CYP1A1以及白细胞介素(IL)-4的基因多态性与三氯乙烯(TCE)药疹样皮炎易感性的关系.方法 选择35例TCE药疹样皮炎病例作为病例组,选无皮肤损害的35名健康工人作为对照组.应用实时荧光定量聚合酶链反应(PCR)和TaqMan MGB探针技术,检测病例组和对照组CYP2E1、CYP1A1和IL-4基因的单核苷酸多态性(SNP),计算病例组和对照组的基因型与等位基因型频率.结果 CYP1A1基因(rs1048943)的SNP多态性检测结果显示,病例组G等位基因频率(37.1%)明显高于对照组,差异有统计学意义(P＜0.01);检测发现,病例组CYP2E1基因-1053 C→岬位点T等位基因频率(41.4%)明显高于对照组,差异有统计学意义(P＜0.01);对IL4基因588 C→岬位点(rs2243250)检测发现,病例组TT纯合突变频率(75.0%)明显高于对照组,差异有统计学意义(P＜0.01),T等位基因频率(87.5%)明显高于对照组,差异有统计学意义(P＜0.01).结论 CYP1A1、CYP2E1和IL-4基因的某些位点的改变可能与少数TCE敏感个体对接触TCE引起的超敏反应存在密切关系,CYP1A1、CYP2E1和IL-4的基因多态性可能是TCE药疹样皮炎患者易感性差异相关的遗传学因素之一.

Relationship between gene polymorphism of CYP2E1,CYP1A1,IL-4 and medicamentosa-like dermatitis induced by trichloroethene

Objecfive To investigate the relationship between gene polymorphism of CYP2E1,CYP1A1,IL-4 and susceptibility of medieamentosa-like dermatitis induced by trichloroethylene(TCE).Methods 35 patients with medicamentosa-like dermatitis induced by TCE were chosen as the patient group,and 35 healthy workers as control group.The real-time quantitative polymerase chain reaction(PCR)with TaqMan minor groove binding (MGB) probes was used to test single nucleotide polymorphisms(SNP)of CYP2E1,CYP1A1 and IL-4 in the patients with medieamentosa-like dermatitis as well as in the control.The genotypes and the frequeney of genotype or allele were compared between the patients and control with statistical analysis.Results The frequency of allele G within CYP1A1 gene(rs1048943)was significantly higher in TCE patients(37.1%)than that in control(P＜0.05);the frequency of allele T within CYP2E11053 C/T Was significandy higher in TCE patients(41.4%)than that in control(P＜0.01);the frequency of T/T within IL-4-588 C/T(rs2243250)was significantly higher in TCE patients(75.0%)than that in control (P＜0.01).and the frequency of allele T within IL-4-588 C/T(rs2243250)was also significantly higher in TCE patients(87.5%)than that in control(P＜0.01).Conclusion The gene polymorphism of CYP2E1,CYP1A1.IL-4 is probably associated with hypersensitivity for the TCE patients with medicamentosa-like deliRatitis.and could be one of the genetic factors related tO the individual susceptibility to TCE exposure.