血管紧张素Ⅰ转换酶基因多态性与2型糖尿病肾病的关联性

目的：探讨血管紧张素I转换酶(ACE)基因多态性与2型糖尿病肾病的关系。方法：2型糖尿病并发肾病组（DN）患者80例，单纯2型糖尿病组（DM）患者60例，60名健康人作对照（正常对照组），抽提每人外周血中白细胞基因组DNA，采用聚合酶链反应（PCR）扩增ACE基因第16 内含子的一个287 bp Ａlu的插入/缺失（I/D） 基因片段,进行1.5%的琼脂糖凝胶电泳,并在紫外线灯下观察荧光带并确认每例的基因型。各组间的基因型频数分布应用Hardy-Weinberg遗传平衡定律检验和χ²检验。等位基因频数分布应用χ²检验。结果：ACE 基因的3种基因型II、ID和DD的频数分布符合Hardy-Weinberg平衡定律；各组间ACE 基因的基因型频数分布比较差异无显著性（χ² =1.10P>0.05）；3组的等位基因I和D的频数分布比较差异无显著性（χ² =0.84，P>0.05）。结论：ACE 基因多态性与2型糖尿病肾病的发病无关联性。

Expressions of CD40 and ICAM-1 in primary hepatocarcinoma and clinical significance

Objective To investigate the expressions of CD40 and ICAM-1 in primary hepatocarcinoma and clinical significance and analyze their relationships with carcinogenesis and progression of liver cancer. Methods S-P immunohistochemistry was used to detect the expressions of CD40 and ICAM-1 in 40 cases of hepatocarcinoma and 25 cases of normal liver tissue (distance from cancer tissues≥2 cm).Results The positive expression rates of CD40 and ICAM-1 were 47.5% and 82.5% in liver cancer tissue,which was significantly higher than those in normal liver tissue (P<0.01).The positive expression rate of CD40 in liver cancer tissue was associated with lymph node metastasis(P<0.05),but not associated with histological grade(P>0.05).The positive expression rate of ICAM-1 was associated with lymph node metastasis as well as histological grade(P<0.01 or P<0.05). Conclusion There are abnormal expressions of CD40 and ICAM-1 in liver cancer.It may be a reliable indicator to judge the invasion and metastasis and prognosis of primary hepatocarcinoma and provide experimental evidence for biological and clinical research.