锚定表达GM-CSF疫苗的抑瘤效果研究

子宫颈癌是妇科比较常见的恶性肿瘤之一,其病因及发病机制不清.近年来,随着细胞分子生物学研究的深入,发现肿瘤的发生发展不仅取决于细胞的增殖速率,而且与细胞凋亡有一定关系,本实验通过对宫颈癌组织芯片进行凋亡相关基因bag-1检测,探讨宫颈癌的发病机制.Bag-1是一种已经被确认的多功能结合蛋白,具有抗细胞凋亡的能力,该蛋白质为一种由219个氨基酸残基组成的酸性蛋白质.

The Antitumor Effects of Tumor Vaccine with Expression of Granulocyte-Macrophage Colony-Stimulating Factor Anchored on Mouse Melanoma Tumor Cell Surface

Objective:To investigate the vaccine potency of GM-CSF anchored B16 tumor cells. Methods:In this study, mGM-CSF was expressed on surface of B16 mice melanoma cells by GPI-modifying. C57BL/6 mice were inoculated with GM-CSF anchored cells and wide-type B16 cells to evaluate whether the GM-CSF anchored cells could elicit a protective and systemtic antitumor response. Results:GM-CSF anchored cells resulted in remarkable loss of tumorgenicity in syngenetic mice. The tumor occurrence rate of GM-CSF anchored B16 cells was 58.8% on C57BL/6 mice with 1×10 6- B16 cells/mice inoculated (n=12) and that of wide-type B16 cells was 100%, The C57BL/6 mice receiving inoculation with 5×10 5 GM-CSF anchored cells/mice never grew tumor. These mice were challenged with wide-type B16 cells, and only a minority of mice grew tumor after wide-type B16 cells inoculation. Conclusion: GM-CSF protein anchored cells could elicit a protective and systemtic antitumor responses.