液相色谱-电喷雾离子阱质谱法研究人尿中1,5-二咖啡酰奎宁酸的代谢产物

目的 研究健康受试者口服1,5-二咖啡酰奎宁酸后尿液中的代谢产物.方法 健康受试者每人口服1,5-二咖啡酰奎宁酸600 mg,收集0-24 h的尿样,经C_(18)小柱固相萃取纯化后,用液相色谱-电喷雾离子阱质谱联用技术对人尿中的代谢产物进行分析鉴定.结果 在人尿中发现了1,5-二咖啡酰奎宁酸的甲基化、葡萄糖醛酸化及甲基-葡萄糖醛酸化代谢产物共28个.其中,有2个代谢产物结构经标准品对照得到确证.结论 甲基化、葡萄糖醛酸化和异构化反应是1,5-二咖啡酰奎宁酸在人体内的3种重要代谢途径.

Study on the metabolites of 1, 5-dicaffeoylquinic acid in human urine by LC-ESI/MS/MS assay

Objective To investigate metabolites of 1,5-dieaffeoylquin-ie acid (1,5-DCQA) in human urine after oral administration of 1, 5-DCQA. Methods Urine samples were collected 0-24 h after the oral administration of 600 mg dose of 1,5-DCQA, then the samples were pu-rified through C_(18) solid-phase extraction cartridge. The purified samples were analyzed by liquid chromatography and tandem electrospray ioniza-tion ion trap mass spectrometry (LC-ESI/MS/MS). Results A total of 28 metabolites were discovered, including methylated, glucuronidated and methyl-glucuronidated metabolites. Two important metabolites were determined by comparing with synthesized standards. Conclusion These results suggest that methylation, glucuronidation and isomerization are three important metabolic pathways of 1, 5-DCQA in human.