Peripheral-type benzodiazepine receptor (PBR) gene amplification in MDA-MB-231 aggressive breast cancer cells |
| |
Authors: | Hardwick Matthew Cavalli Luciane R Barlow Keith D Haddad Bassem R Papadopoulos Vassilios |
| |
Affiliation: | Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Georgetown, Washington DC 20007, USA. |
| |
Abstract: | Recent studies using human breast cancer cell lines, animal models, and human tissue biopsies have suggested a close correlation between the expression of the peripheral-type benzodiazepine receptor (PBR) and the progression of breast cancer. This study investigates the genetic status of the PBR gene in two human breast cancer cell lines: MDA-MB-231 cells, which are an aggressive breast cancer cell line that contains high levels of PBR, and MCF-7 cells, which are a nonaggressive cell line that contains low levels of PBR. Both DNA (Southern) blot and fluorescence in situ hybridization analyses indicate that the PBR gene is amplified in MDA-MB-231 relative to MCF-7 cells. These data suggest that PBR gene amplification may be an important indicator of breast cancer progression. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|