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| 卡介苗、卡介苗多糖核酸对肺炎大鼠肺Toll样受体mRNA的影响 | |
| 引用本文： | 侯松萍,董震,于倩.卡介苗、卡介苗多糖核酸对肺炎大鼠肺Toll样受体mRNA的影响[J].山西医科大学学报,2008,39(10). |
| 作者姓名： | 侯松萍 董震 于倩 |
| 作者单位： | 1. 吉林大学中日联谊医院呼吸科,长春,130033 2. 吉林大学中日联谊医院耳鼻咽喉科 3. 吉林大学中日联谊医院药剂科 |
| 摘 要： | 目的探讨卡介苗多糖核酸（BCG-PSN）、卡介苗（BCG）对肺炎大鼠肺TLR2、TLR4mRNA表达的影响。方法57只大鼠随机分为肺炎模型组（PM组，n=51）及健康对照组（HC组，n=6）．第4天PM组随机抽取6只（非吸入组，NI组）进行相关检查确定肺炎模型是否制作成功。然后肺炎模型组45只肺炎大鼠再随机分成生理盐水组（NS组）、卡介苗组（BCG组）、卡介苗多糖核酸组（BCG-PSN组）。每组又按吸入次数分为3次、5次、7次组。采用RT-PCR方法研究肺炎大鼠肺TLR2mRNA、TLR4mRNA的表达，以及肺炎时雾化吸入前后肺TLR2mRNA、TLR4mRNA表达。结果肺炎第4天，肺TLR2mRNA、TLR4mRNA表达与HC组相比无统计学差异。吸入BCG-PSN5次时，TLR2mRNA表达与HC组相比P〈0．05，7次时P〈0．01；与NI组及NS组相比吸入BCG-PSN7次时P〈0．05。吸入BCG3次时，TLR2mRNA表达与HC组、NI组、NS组相比，P〈0．05，吸入5次、7次时，P〉0．05。同组间吸入BCG或BCG-PSN5次，7次时TLR4mRNA表达略增强，但无统计学差异（P〉0．05）。结论BCG、BCG-PSN能使肺炎大鼠肺TLR2mRNA表达明显增强，但BCA3-PSN较BCG起效慢、作用强、持续时间长，而且也不能使肺炎大鼠肺TLR4mRNA表达增强。 |
| 关 键 词： | BCG BCG—PSN 支气管肺炎 TLR2 TLR4 |
Effect of BCG or BCG-PSN by inhalation on expression of TLR-2 and TLR-4 gene in rats with bronchopneumonia in lung |
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| HOU Song-ping,DONG Zhen,YU Qian.Effect of BCG or BCG-PSN by inhalation on expression of TLR-2 and TLR-4 gene in rats with bronchopneumonia in lung[J].Journal of Shanxi Medical University,2008,39(10). | |
| Authors: | HOU Song-ping DONG Zhen YU Qian |
| Institution: | 1Dept of Respiratory Medicine;China-Japan Union Hospital;Jilin University;Changchun 130033;China;2Dept of Otolaryngology;3Dept of of Pharmacy;Jilin University |
| Abstract: | Objective To compare the effect of BCG and BCG-PSN by inhalation on endogenous gene expression of TLR2,TLR4 in lung of rats with bronchopneumonia.Methods Fifty-seven male SD rats were randomly divided into:pneumonia model group(PM group,n=51),health control group(HC group,n=6).At day 4 after injecting LPS in PM group,six rats(PM-NI group)were randomly selected and used to detect whether the bronchopneumonia rat model was established successfully.Then 45 rats in PM group were randomly divided into 3 groups:n... |
| Keywords: | BCG BCG-PSN bronchopneumonia TLR2 TLR4 |
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