| 慢性缺氧对肺动脉内皮细胞Ⅲ型一氧化氮合酶mRNA表达的影响 |
| |
| 引用本文： | 孔炜,王迪浔. 慢性缺氧对肺动脉内皮细胞Ⅲ型一氧化氮合酶mRNA表达的影响[J]. 中国病理生理杂志, 2002, 18(3): 262-264 |
| |
| 作者姓名： | 孔炜 王迪浔 |
| |
| 作者单位： | 华中科技大学同济医学院, 湖北武汉 430030 |
| |
| 基金项目： | 国家自然科学基金重点资助项目 (No .39730 1 90 ) |
| |
| 摘 要： | 目的和方法:采用原位杂交技术结合图象分析检测常氧(PO221.3kPa)及慢性缺氧(PO25.3±0.7kPa)培养的猪肺动脉内皮细胞Ⅲ型一氧化氮合酶mRNA的表达及其对急性缺氧刺激反应的变化。结果:常氧条件培养的肺动脉内皮细胞急性缺氧12h,NOSⅢmRNA表达明显增加(2、4、6代分别比缺氧前增加48%、125%、119%,P＜0.05);慢性缺氧条件下培养的肺动脉内皮细胞急性缺氧12h,NOSⅢmRNA表达亦明显增加(分别比急性缺氧前增加92%、245%、565%,P＜0.01)。其增加的幅度在慢性缺氧培养组均高于同代常氧组,且随着缺氧时间的延长,这种差别更显著。结论:慢性缺氧增强了肺动脉内皮细胞Ⅲ型一氧化氮合酶基因对急性缺氧的反应,这可能在慢性缺氧时肺血管对缺氧的反应性降低中,具有重要作用。
|
| 关 键 词： | 低氧 一氧化氮合酶 内皮 细胞 |
| 文章编号： | 1000-4718(2002)03-0262-03 |
| 收稿时间： | 2000-11-13 |
Effects of chronic hypoxia on expression of NOSⅢ mRNA induced by acute hypoxia in pulmonary artery endothelial cells |
| |
| KONG Wei,WANG Di xun. Effects of chronic hypoxia on expression of NOSⅢ mRNA induced by acute hypoxia in pulmonary artery endothelial cells[J]. Chinese Journal of Pathophysiology, 2002, 18(3): 262-264 |
| |
| Authors: | KONG Wei WANG Di xun |
| |
| Affiliation: | Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| Abstract: | AIM and METHODS:Insitu hybridization and image-analysis system were used to investigate the expression of NOSⅢ mRNA in cultured porcine pulmonary artery endothelial cells (PAEC) under normoxic or chronic hypoxic conditions. RESULTS:The expression of NOSⅢ mRNA were increased by 48%, 125%, 119% respectively in the 2nd, 4th, 6th subculture of normoxic PAEC after expouse to acute hypoxia for 12 hours while in chronic hypoxic group,the expression of Ⅲ NOS mRNA were increased by 92%, 245%, 565% separately. The increase was more apparent in chronic hypoxic group than in normoxic group and became more significant following the prolonging duration of hypoxia. |
| |
| Keywords: | Anoxia Nitric oxide synthase Endothelium Cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 |
|
点击此处可从《中国病理生理杂志》下载全文 |
|
