| 甘草中黄酮的纯化方法和含量测定 |
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| 引用本文： | 李俊松,徐德生,冯怡,陈丽华.甘草中黄酮的纯化方法和含量测定[J].中成药,2007,29(7):997-1000. |
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| 作者姓名： | 李俊松 徐德生 冯怡 陈丽华 |
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| 作者单位： | 上海中医药大学,上海,201203 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 目的:研究聚酰胺富集甘草中黄酮的工艺参数,同时建立其含量测定方法。方法:以二氢黄酮类化合物在碱性条件下转化为查尔酮类的量作为指标,以柚皮苷为对照品,经聚酰胺吸附柱富集后,采用碱性比色法显色、测定。结果:纯化工艺条件为甘草聚酰胺比2∶1(g/g),树脂径高比1∶7,用5倍量柱体积70乙醇洗脱,甘草黄酮洗脱率在90左右;含量测定平均加样回收率为98.81(RSD=2.00)。总固物中黄酮含量可达45。结论:本法富集、纯化甘草总黄酮可行;甘草黄酮含量测定方法稳定,可靠。
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| 关 键 词： | 甘草 黄酮 含量测定 聚酰胺 |
| 文章编号： | 1001-1528（2007）07-0997-04 |
| 修稿时间： | 2006-04-15 |
Purification and assay of flavonoids in glycyrrhiza |
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| LI Jun-song,XU De-sheng,FENG Yi,CHEN Li-hua.Purification and assay of flavonoids in glycyrrhiza[J].Chinese Traditional Patent Medicine,2007,29(7):997-1000. |
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| Authors: | LI Jun-song XU De-sheng FENG Yi CHEN Li-hua |
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| Institution: | Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | AIM:To investigate the process parameters of adsorbing and separating the total flavones of Radix et Rhizoma glycyrrhiae and its assay. METHODS: Chalcone that flavanone in Licorice root was converted into in alkaline condition was adopted as index and niaringoside as control,through polyamide treatment,alkaline colorimetty was used to determine chalcone content. RESULTS: Under the condtion,namely, ratio of quantity of Licorice root to polyamide was 2:1,ratio of column diameter to column heighth was 1:7,70%ethanol used to eluate,flavone extract was up to 45%with 90%eluting yield.The recoveny of assaying was 98.17%(RSD=2.00%). CONCLUSION: The purification and assay of Licorice flavonoids are reliable and efficacious. |
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| Keywords: | Glycyrrhiza flavone polyamide |
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