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| HPLC-MSn分析扶正化瘀方中五味子的入血成分 | |
| 引用本文： | 沈丹萍,杨莉,陶艳艳,王清兰,刘成海. HPLC-MSn分析扶正化瘀方中五味子的入血成分[J]. 中国中药杂志, 2011, 36(7): 854-858 |
| 作者姓名： | 沈丹萍 杨莉 陶艳艳 王清兰 刘成海 |
| 作者单位： | 1. 华东理工大学,药学院,上海,200237;上海中医药大学,附属曙光医院肝病研究所,上海,201203 2. 上海中医药大学,中药研究所中药,标准化教育部重点实验室暨中药新资源与品质评价国家中医药管理局重点研究室,上海,201203 3. 上海中医药大学,附属曙光医院肝病研究所,上海,201203 4. 华东理工大学,药学院,上海,200237;上海中医药大学,附属曙光医院肝病研究所,上海,201203;上海高校中医内科学E研究院,上海,201203 |
| 基金项目： | 国家重点基础研究发展计划(973)项目(2006CB504801);上海市自然科学基金项目(10ZR1431000);上海市教育委员会E-研究院建设项目(E-03008) |
| 摘 要： | 目的:分析扶正化瘀方(Fuzheng Huayu decoction,FZHY)灌胃大鼠后进入血液的五味子中木脂素类成分,以探讨部分体内效应成分.方法:应用液相色谱-电喷雾质谱联用(HPLC-ESI-MSn)技术,通过空白血清,口服FZHY方后含药血清,FZHY方和对照品之间的色谱图和质谱碎片信息的对比分析,结合文献报道,确定口服FZHY方后大鼠的入血成分.结果:FZHY方和口服FZHY方后含药血清中均含有五味子醇甲、五味子醇乙、五味子酯甲、五味子乙素,并在FZHY方中还发现含有五味子甲素和丙素.结论:五味子醇甲、五味子醇乙、五味子酯甲、五味子乙素是直接吸收入血的成分,可能是发挥抗肝纤维化的体内效应成分. |
| 关 键 词： | 扶正化瘀方 木脂素类成分 五味子 含药血清 抗肝纤维化 |
| 收稿时间： | 2010-09-08 |
Identification of the lignans components after oral administration of Fuzheng Huayu decoction in rat serum by HPLC-MSn |
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| SHEN Danping,YANG Li,TAO Yanyan,WANG Qinglan and LIU Chenghai. Identification of the lignans components after oral administration of Fuzheng Huayu decoction in rat serum by HPLC-MSn[J]. China Journal of Chinese Materia Medica, 2011, 36(7): 854-858 | |
| Authors: | SHEN Danping YANG Li TAO Yanyan WANG Qinglan LIU Chenghai |
| Affiliation: | School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;E-Institute of Traditional Chinese Medicine Internal Medicine, Shanghai Municipal Education Commission, Shanghai 201203, China |
| Abstract: | Objective : To study the lignans components in rat serum after oral administration of Fuzheng Huayu decoction (FZHY), and to investigate the active ingredients in vivo. Method : A rapid, sensitive and selective method using liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn)was established. The serum samples were extracted with ethyl acetate (EtOAc)for three times. The chromatographic separation was achieved on a Waters Atlantis T3 column by gradient elution using methanol and water containing 5 mmol·L-1 ammonium acetate as mobile phase, at a flow rate of 0.2 mL·min-1. Mass spectra were acquired in positive ion mode. Identification and structural elucidation of the components in FZHY and dosed serum were performed by comparing their retention time and MSn spectra with those of reference compounds and reported data in the literatures. Result : Schisandrin, schisandrol B, schisantherin A and schisandrin B were found in FZHY and dosed serum,but schisandrin C and deoxyschizandrin were only found in FZHY. Conclusion : Schisandrin, schisandrol B, schisantherin A and schisandrin B can be directly absorbed into the blood after oral administration of FZHY, and the four lignans components from Schisandra chineisis might play a key role as the ingredient basement of FZHY for anti-liver fibrosis. |
| Keywords: | Fuzheng Huayu decoction lignans Schisandra chineisis serum containing drug anti-liver fibrosis |
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