| 糖皮质激素对吗啡依赖大鼠海马c-fos基因表达的影响 |
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| 引用本文： | 任波,王新华,石学银.糖皮质激素对吗啡依赖大鼠海马c-fos基因表达的影响[J].解放军药学学报,2004,20(4):245-248. |
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| 作者姓名： | 任波 王新华 石学银 |
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| 作者单位： | 中国人民解放军第302医院,麻醉室,北京,100039;中国人民解放军第二军医大学长征医院,麻醉科,上海,200003 |
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| 基金项目： | 国家自然科学基金项目 ,No .3 9870 75 7 |
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| 摘 要： | 目的 观察吗啡依赖大鼠脑内海马区c-fos mRNA水平的变化，研究糖皮质激素(地塞米松)控制阿片戒断症状的分子机理。方法 剂量递增腹腔注射吗啡建立大鼠成瘾模型，给与地塞米松干预，纳洛酮促瘾后观察戒断症状，应用原位杂交方法观察海马区c-fos mRNA。结果 吗啡依赖大鼠经地塞米松干预后，由纳洛酮催促戒断症状评分明显低于未经地塞米松处理组；同时经地塞米松干预后，海马c-fos mRNA的表达量明显低于未经地塞米松处理，但较盐水对照组高。结论糖皮质激素(地塞米松)能抑制吗啡依赖大鼠戒断症状以及海马c-fos mRNA的表达。
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| 关 键 词： | 糖皮质激素 吗啡依赖 c-fos基因表达 大鼠 |
| 文章编号： | 1008-9926(2004)04-0245-04 |
| 修稿时间： | 2003年8月25日 |
Effect of Glucocorticoid on the Changes of c-fos mRNA Expression of Morphine Dependent Rats |
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| Abstract: | Aim To explore between the molecular mechanism of glucocorticoid in the treatment of opioid withdrawal syndromes and the changes of c-fos mRNA expression.method Rats were administered with morphine by interaperitioneal injection with does adds to develop addiction mode.Twenty-four rats were randomly assigned into three groups and received different kinds of treatment (Group one:morphine,ip.Group two:morphine,dexamethasone,ip.Group three:saline,ip).Naloxone was administrated to precipitate withdrawal syndromes.In situ hybridization histochemistry (ISHH) was proceeded in hippocampus detect the changes of c-fos mRNA expression.Result The withdrawal syndromes in Group two were alleviated better than that of Group one,and was higher than that of Group three.Conclusion Glucocorticoid produces suppressing effect on the withdrawal syndromes of morphine dependent rats when the increasing expression of c-fos mRNA in hippocampus is inhibited. |
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| Keywords: | Glucocorticoid Morphine dependent c-fos gene Rat |
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