| Abstract: | Objective To study abnormalities of Rb gene in the initiation of oral squamous cell carccinoma (SCC), and the relationship between deletion of Rb gene and some clinico pathological parameters. Methods DIG High Prime labeled Rb 3 6 kb DNA probe was used to detect 28 cases of oral squamous cell carcinomas digested by restriction endonuclease EcoRI using Southern blotting technique. 15 males and 13 females, aged from 34 to 69 patients were enrolled in this study. Results The structural abnormal alterations of Rb gene were found in 7 cases of total samples:6 cases with deletion of 5.4, 4.1 and 2.3 kb fragments and one case was rearrangement. No correlation was found with sex, age, site of primary tumor, level of histopathological differentiation and TNM staging on further analyses. Conclusion The abnormalities of Rb gene may be associated with carcinogenesis of oral SCC. |
