雷帕霉素对培养的人Tenon′s囊成纤维细胞的作用

目的：观察雷帕霉素(rapamycin)对培养的人Tenon′s囊成纤维细胞的增殖及细胞周期的作用。方法：将培养的人Tenon′s囊成纤维细胞用含不同雷帕霉素浓度的培养液培养24h后, 用MTT法测定细胞的增殖情况，用流式细胞仪测定细胞周期的变化情况。结果：用含10、20、40、80和160ng/ml雷帕霉素的培养液培养细胞时，细胞增殖抑制率分别为9.32%，22.55%，41.42%，42.25%，43.57%。细胞周期分析显示，雷帕霉素(40ng/ml)作用后，G0/G1期细胞较对照组增多(41.76% vs 44.19%，P＜0.05)，S期细胞较对照组减少(45.42%　vs　40.18%，P＜0.05)。结论：雷帕霉素具有抑制人Tenon′s囊成纤维细胞增殖的效果，且随浓度增加抑制作用增强，并将细胞抑制在G1期的限制点内。

Role of rapamycin on cultured human Tenon's capsule fibroblasts

Objective: To explore the effect of rapamycin on the proliferation and differentiation of human Tenon′s capsule fibroblasts in cell cycles. Methods: The cultured fibroblasts were treated in DMEM medium containing various concentrations of rapamycin for 24 hours. The growth of fibroblasts was measured by a methylthiazlyl tetrazolium (MTT) assay. The flow cytometry method was used to evaluate the cell cycles. Results: Proliferation of fibroblasts were suppressed by rapamycin from a dose of 10?ng/ml to 160ng/ml(P＜0.05)． The cell growth inhibition rate treated with rapamycin concentrations of 10ng/ml, 20ng/ml, 40ng/ml, 80ng/ml, and 160ng/ml were 9.32%, 22.55%, 41.42%, 42.25%, and 43.57% respectively. The cell population was significantly increased at the G0/Gl phase from 41.76% to 44.19% and decreased at the S phase from 45.42% to 40.18% after the rapamycin treatment． Conclusion: Rapamycin is a potential inhibitor that blocks the proliferation of human Tenon′s capsule fibroblasts at the Gl restriction point．