Collagen mutation causes changes of the microdamage morphology in bone of an OI mouse model |
| |
| Authors: | X. Neil Dong Mahyar Zoghi Qitao Ran Xiaodu Wang |
| |
| Affiliation: | 1. New York University School of Medicine NY, United States;2. The City College of New York, NY;3. The Royal College of Surgeons in Ireland, Ireland;1. Department of Health and Kinesiology, The University of Texas at Tyler, Tyler, TX 75799, USA;2. Department of Electrical Engineering, The University of Texas at Tyler, Tyler, TX 75799, USA;3. Department of Mechanical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USA;1. Musculoskeletal Biology and Mechanics Lab, Department of Basic Medical Sciences, Purdue University, IN, USA;2. School of Mechanical Engineering, Purdue University, IN, USA;3. Weldon School of Biomedical Engineering, Purdue University, IN, USA;4. Department of Aerospace and Mechanical Engineering, University of Notre Dame, IN, USA;1. Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, SK, Canada;2. Department of Anatomy & Cellular Biology, University of Saskatchewan, Saskatoon, SK, Canada;3. College of Kinesiology, University of Saskatchewan, Saskatoon, SK, Canada;1. Department of Nephrology, The Royal Melbourne Hospital, Parkville, Australia;2. Department of Medicine (RMH), University of Melbourne, Parkville, Australia;3. Department of Renal Medicine, Westmead Hospital, Westmead, Australia;4. Osteoporosis and Bone Biology Division, Garvan Institute of Medical Research, Darlinghurst, Australia;5. Department of Radiology, The Royal Melbourne Hospital, Parkville, Australia;6. Monash University, Clayton, Australia;7. Departments of Radiology and Orthopaedic Surgery, University of Pennsylvania, PA, USA;1. μVIS X-ray Imaging Centre, Faculty of Engineering and the Environment, University of Southampton, SO17 1BJ Southampton, UK;2. Bioengineering Sciences Research Group, Faculty of Engineering and the Environment, University of Southampton, SO17 1BJ Southampton, UK;3. Institute for Lightweight Design and Structural Biomechanics, Vienna University of Technology, 1040 Vienna, Austria |
| |
| Abstract: | Previous studies have postulated that ultrastructural changes may alter the pattern and capacity of microdamage accumulation in bone. Using an osteogenesis imperfecta (OI) mouse model, this study was performed to investigate the correlation of collagen mutation with the microdamage morphology and the associated brittleness of bone. In this study, femurs from mild OI and wild type mice were fatigued under four-point bending to create microdamage in the specimens. Then, the microdamage morphology of these specimens was examined using the bulk-staining technique with basic fuchsin. Similar with the results of previous studies, it was observed that linear microcracks were formed more easily in compression, whereas diffuse damage was induced more readily in tension for both wild-type and mild-type mice. However, less diffuse damage was found in the tensile side of mild OI mouse femurs (collagen mutation) compared with those of wild type mice, showing that the microdamage morphology is correlated to the brittleness of bone. The results of this study provide direct evidence that supports the prediction made by the previous numerical simulation studies, suggesting that microdamage morphology in bone is significantly correlated with the integrity of the collagen phase. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
