Impact of radiotherapy and chemotherapy on biomarkers of oxidative DNA damage in lung cancer patients |
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| Authors: | Marika Crohns Seppo Saarelainen Marina Erhola Hannu Alho Pirkko Kellokumpu-Lehtinen |
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| Institution: | 1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia;2. Laboratory for Personalized Medicine, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia;3. Department of Clinical Pathology, Clinical Hospital Dubrava, Avenija Gojka Suska 6, 10000 Zagreb, Croatia;4. School of Medicine, University of Zagreb, Salata 3, 10000 Zagreb, Croatia;5. Laboratory for Molecular Endocrinology and Transplantation, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia |
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| Abstract: | ObjectivesTo assess oxidative damage to DNA during lung cancer (LC) treatments.Design and methodsUrinary levels of 8-oxoguanine (8-oxoGua) and levels of 8-oxo-2′-deoxyguanosine (8-oxodG) from urine and whole blood were determined in 36 non-cancer controls and 65 LC patients before any treatments. Samples were also obtained of LC patients during and after radiotherapy (RT, n = 33) and chemotherapy (CT, n = 16).ResultsStage IV LC patients had higher urinary 8-oxoGua and 8-oxodG levels than patients with stage I–III disease (p = 0.044 and p = 0.034, respectively). Urinary 8-oxodG levels increased during the first week of RT (p < 0.001). Nuclear 8-oxodG increased during RT and 3 months after start of RT. Nuclear 8-oxodG levels also rose between the first two CT cycles (p = 0.043), and urinary 8-oxodG levels during the sixth CT cycle (p = 0.009).ConclusionsUrinary DNA damage biomarker levels may be associated with LC stage. Both RT and CT increase the parameters of DNA oxidation. |
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