Bimodal distribution of osteocyte lacunar size in the human femoral cortex as revealed by micro-CT |
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| Authors: | KM Hannah CDL Thomas JG Clement F De Carlo AG Peele |
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| Institution: | 1. School of Mathematical Sciences, Monash University, Clayton, VIC 3800, Australia;2. St Vincent''s Institute of Medical Research, The University of Melbourne, Fitzroy, VIC 3065, Australia;1. Department of Bioengineering, Imperial College London, UK;2. Institute for Biomechanics, ETH Zürich, Switzerland;3. Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Germany;4. Institute of Pathology, University Medical Center Hamburg-Eppendorf, Germany;1. iNANO and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark;2. Department of Biomedicine, Aarhus University, Aarhus, Denmark;1. Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri, Kansas City, 615 E. 25th Street, Kansas City, MO 64108, USA;2. Biomedical Sciences Department, Oakland University William Beaumont School of Medicine, 414 O''Dowd Hall, Rochester MI, 48309, USA;3. Medical Physiology Department, Assiut University School of Medicine, 71516 Asyut, Egypt;4. Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry, 3302 Gaston Ave., Dallas, TX 75246, USA |
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| Abstract: | Tomographic reconstructions of sections of human femoral bone were created from x-ray data sets taken using synchrotron radiation of 26.4 keV and with isotropic voxels 1.47 μm on a side. We demonstrate that it is possible to segment the data to isolate both the osteocyte lacunae and the Haversian canals in the bone as well as identifying osteon boundaries. From this information a wealth of data relating to bone structure becomes available. The data were used to map the spatial positions of the osteocyte lacunae, relative to the Haversian canals and of the osteon boundaries. The dimensions and volume of the imaged osteocyte lacunae were measured for close to 10,000 lacunae. When averaged over the 11 osteons measured, osteocyte densities varied from 4 × 104 per mm3 close to the Haversian canals to about 9 × 104 per mm3 at 80% of osteon radius. The nearest-neighbour distances varied from 10 μm to 40 μm with a peak at 23 μm and an approximately normal distribution. The distribution of lacunar long-axis length was also approximately normal with a small positive skew and the peak value was 8 μm with a range from 3 μm to 20 μm. The most significant finding from this study was that the distribution of the measured volumes of osteocyte lacunae had two distinct peaks, one at 200 μm3 and a second at 330 μm3. |
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