2016-2019年常州地区无偿献血者酶免和核酸平行检测结果分析

目的 分析ELISA和NAT平行的血液筛查模式对降低经输血感染病原体风险的有效性.方法 收集常州市2016—2019年270215例无偿献血者血液标本,采用两次ELISA并行检测HBsAg、抗HCV、抗HIV和抗TP.268264例ELISA双阴性标本采用6人份混样(pool)NAT进行HBV DNA、HCV RNA和...

Analysis of the Results of Enzyme Immunoassay and Nucleic Acid Parallel Testing of Unpaid Blood Donors in Changzhou from 2016 to 2019

Objective To analyze the effectiveness of the parallel blood screening mode of ELISA and NAT in reducing the risk of pathogen infection through blood transfusion.Methods The blood samples of 270215 unpaid blood donors in Changzhou from 2016 to 2019 were collected.Two ELISAs were used to detect HBsAg,anti-HCV,anti-HIV and anti-TP in parallel.268264 double-negative ELISA specimens were tested for HBV DNA,HCV RNA,and HIV RNA using 6 pools of NAT,and the pool of nucleic acid-positive samples was split and single-tested.Results The positive rates of HBsAg,anti-HCV,anti-HIV and anti-TP of 270215 unpaid blood donors were 2.58‰(697 cases),1.49‰(402 cases),0.23‰(61 cases)and 3.06‰(827 cases),respectively.The positive rates of HBV DNA,HCV RNA and HIV RNA in 268264 unpaid blood donors with negative enzyme immunity were 0.86‰(230 cases),0.01‰(3 cases)and 0.01‰(2 cases),respectively.Conclusion The two detection methods of ELISA and NAT can complement each other,greatly reduce the residual risk of pathogens infected by blood transfusion and ensure the safety of blood transfusion.NAT can further shorten thewindow periodfor the detection of blood infectious viruses and detect concealed viruses.