Development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum |
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| Authors: | Qin Xiao Haifang Li Guomao Hu Hongrui Wang Zhenjia Li Jin-Ming Lin |
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| Affiliation: | 1. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China;2. Beijing Chemclin Biotech Co., Ltd. Beijing Academy of Science and Technology, Haidian, Beijing 100094, China;1. School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun 130000, China;2. School of Media and mathematics&Physics, Jilin Engineering Normal University, Changchun 130012, China;3. Changchun Normal University, Changchun 130000, China;4. College of Physics, Jilin University, Changchun 130012, China;1. Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia;2. Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia;3. Chemical Engineering Discipline, School of Engineering, Monash University, Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia |
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| Abstract: | ObjectivesA rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated.Design and methodsThe method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)–luminol–H2O2 chemiluminescent system was chosen as the detection system.ResultsThis method had a detection limit of 0.2 mIU/mL and a linear range of 0.5–200 mIU/mL. The assay showed little cross-reactivity, and the intra- and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA).ConclusionThe MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis. |
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