| 甲地孕酮与三苯氧胺联合应用对乳腺癌MCF-7细胞生长和凋亡的影响 |
| |
| 引用本文： | 史玉霞,韩 克,王雪晨.甲地孕酮与三苯氧胺联合应用对乳腺癌MCF-7细胞生长和凋亡的影响[J].南京医科大学学报,2009,29(8):1067-1070. |
| |
| 作者姓名： | 史玉霞 韩 克 王雪晨 |
| |
| 作者单位： | 南京医科大学鼓楼临床医学院妇产科,江苏,南京,210029
|
| |
| 基金项目： | 南京市科技发展指导性计划 |
| |
| 摘 要： | 目的:探讨小剂量甲地孕酮(MA)和三苯氧胺(TAM)联合应用对乳腺癌细胞MCF-7生长和凋亡的影响.方法:分别或联合应用TAM、MA作用于MCF-7细胞,应用四甲基偶氮唑蓝(MTT)比色法分析细胞生长和抑制作用,应用流式细胞技术测定细胞周期和凋亡率.结果:TAM可抑制MCF-7细胞生长,阻滞细胞周期于G0/G1期,并可诱导细胞凋亡;小剂量的MA对MCF-7细胞生长与凋亡无明显影响,G0/G1.期细胞增多;两者联合应用时,细胞的生长抑制作用加强,凋亡率上升.结论:TAM对MCF-7细胞具有抑制生长和诱导凋亡作用.加用小剂量的MA后,其抑制生长和凋亡诱导作用加强,两者联合应用具有协同作用.
|
| 关 键 词： | 乳腺肿瘤 三苯氧胺 甲地孕酮 细胞周期 凋亡 |
| 收稿时间： | 2009/4/17 0:00:00 |
Effect of tamoxifen and megestrol acetate on growth and apoptosis in MCF-7 breast cancer cell strain in vitro |
| |
| SHI Yu-xi,HAN Ke and WANG Xue-chen.Effect of tamoxifen and megestrol acetate on growth and apoptosis in MCF-7 breast cancer cell strain in vitro[J].Acta Universitatis Medicinalis Nanjing,2009,29(8):1067-1070. |
| |
| Authors: | SHI Yu-xi HAN Ke and WANG Xue-chen |
| |
| Institution: | Department of Gynecology and Obstetrics;the Drum Tower Affiliated Hospital of NJMU;Nanjing 210029;China |
| |
| Abstract: | Objective:To study the effect of tamoxifen (TAM) and megestrol acetate (MA) on breast cancer cell line MCF-7. Methods:Estrogen receptor (ER)-positive MCF-7 human breast cancer cell line was treated by TAM or MA alone,or in combination in vitro. Cell proliferation was evaluated by MTT assay. Distribution of cell cycle and rate of apoptosis were determined by flow cytometry (FCM). Results:TAM can inhibit MCF-7 cells proliferation and induce its apoptosis. The cells were arrested in G0/G1 phase;small doses of ... |
| |
| Keywords: | breast neoplasm tamoxifen megestrol acetate cell cycle apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《南京医科大学学报》浏览原始摘要信息 |
| 点击此处可从《南京医科大学学报》下载免费的PDF全文 |
