| 心肌细胞内钙释放对心肌细胞PTEN表达的影响 |
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| 引用本文： | 周裔忠,祝善俊,于林君,田颖,王江.心肌细胞内钙释放对心肌细胞PTEN表达的影响[J].心脏杂志,2007,19(1):9-11. |
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| 作者姓名： | 周裔忠 祝善俊 于林君 田颖 王江 |
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| 作者单位： | 1. 第三军医大学新桥医院心血管内科,重庆,400037;江西省人民医院心内科,江西,南昌,330006
2. 第三军医大学新桥医院心血管内科,重庆,400037 |
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| 摘 要： | 目的探讨雷尼丁（Ryanodine,RY）介导的心肌细胞内钙释放对PTEN mRNA和蛋白表达影响。方法新生Wistar乳鼠心肌细胞原代培养。用不同浓度的RY（0.0110μmol/L）促进心肌细胞内钙释放,特异的钙敏感指示剂Fura-2/AM测定心肌细胞内钙浓度（Ca2+]i）。逆转录聚合酶链式反应（RT-PCR）、Western blot分别检测PTEN mRNA和蛋白表达。结果RY在0.011μmol/L浓度范围内,能浓度依赖性地促进心肌细胞内钙释放,RY在10μmol/L时,心肌细胞内钙浓度低于RY在1μmol/L时的效应,但仍高于对照组;RY在0.011μmol/L浓度范围内能浓度依赖性促进心肌细胞PTEN mRNA和蛋白表达,RY在10μmol/时,心肌细胞PTEN mRNA和蛋白表达低于1μmol/L的效应,但仍高于对照组。结论RY介导的心肌细胞内钙释放可影响心肌细胞PTEN基因表达,其表达在RY处于0.011μmol/L范围内具有浓度依赖性。
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| 关 键 词： | 钙释放 第10染色体同源丢失性磷酸酶张力蛋白基因 负性调控 心肌细胞 |
| 文章编号： | 1009-7236(2007)01-009-03 |
| 修稿时间： | 2006年2月9日 |
Effects of calcium release induced by Ryanodine on PTEN expression in cardiomyocytes |
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| ZHOU Yi-zhong,ZHU Shan-jun,YU Lin-jun,TIAN Ying,WANG Jiang.Effects of calcium release induced by Ryanodine on PTEN expression in cardiomyocytes[J].Chinese Heart Journal,2007,19(1):9-11. |
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| Authors: | ZHOU Yi-zhong ZHU Shan-jun YU Lin-jun TIAN Ying WANG Jiang |
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| Abstract: | AIM To investigate the effects of calcium release induced by Ryanodine(RY)on phosphatase and tensin homology deleted onchromosome10(PTEN) expression.METHODS Cardiomyocytes were cultured in Wistar rats aged 1-3 days.Intracellular calcium concentration(i) was measured by Fura-2/AM,and PTEN mRNA and protein expression was detected respectively by RT-PCR and Western blot.RESULTS RY increased i of cardiomyocytes,and the PTEN mRNA and protein expression increased with the increase in Ca2 ]i of cardiomyocytes.CONCLUSION Intracellular calcium triggers PTEN expression. |
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| Keywords: | calcium release phosphatase and tensin homology deleted onchromosome10 negative regulate cardiomyocyte |
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