| 利用TCT技术和PCR-RDB法筛查宫颈高度病变 |
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| 引用本文： | 肖克林,吴丽娟,王敏,梅平,王丁,周克元. 利用TCT技术和PCR-RDB法筛查宫颈高度病变[J]. 国际检验医学杂志, 2010, 31(2): 127-128,130 |
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| 作者姓名： | 肖克林 吴丽娟 王敏 梅平 王丁 周克元 |
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| 作者单位： | 深圳市宝安区妇幼保健院检验科,518133;中山大学达安基因股份有限公司科研部,510080;广东省人民医院病理医学部,510080 |
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| 摘 要： | 目的评价TCT技术、PCR-RDB法检测女性宫颈高度病变的价值。方法利用TCT技术和PCR-RDB法分别检测宫颈上皮细胞病变及HPV感染情况,以组织病理检查结果进行评价。结果 TCT技术检测宫颈高度病变的敏感性、特异性、阳性预测值和阴性预测值分别为46.2%(95%CI,19.1%～73.3%)、98.0%(95%CI,96.3%～99.7%)、54.5%(95%CI,25.1%～84.0%)和97.2%(95%CI,95.2%～99.3%),PCR-RDB法分别为100.0%(95%CI,100.0%～100.0%)、58.6%(95%CI,52.5%～64.8%)、11.2%(95%CI,5.5%～17.0%)和100.0%(95%CI,100.0%～100.0%),两者联合后分别为100.0%(95%CI,100.0%～100.0%)、98.0%(95%CI,96.2%～99.7%)、77.3%(95%CI,59.8%～94.8%)和100.0%(95%CI,100.0%～100.0%)。结论 TCT技术和PCR-RDB法检测宫颈高度病变具有很好的互补性,两者联合应用对于筛查宫颈高度病变有很高的应用价值。
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| 关 键 词： | 聚合酶链反应 人乳头瘤病毒16 宫颈肿瘤 |
Detecting high grade cervical intraepithelial neoplasia by TCT and PCR-RDB |
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| Abstract: | Objective To evaluate the diagnostic accuracy of TCT and PCR-RDB in detecting high grade cervical intrepithelial neoplasis.Methods Participants'cervical epithelium specimens were collected for cervical cytology test by TCT and HPV test by PCR-RDB,then,the two methods'accuracy were evaluated by hifftopathology result.Results The sensitivity,specificity,positive predictive value and negative predictive value of TCT for detection≥CIN2 were 46.2%(95%CI,19.1%～73.3%),98.0%(95%CI,96.3%～99.7%),54.5%(95%CI,25.1%～84.0%)and 97.2%(95%CI,95.2%～99.3%),respectively.Those of PCR-RDB were 100.0%(95%CI,100.0%～100.0%),58.6%(95%C1,52.5%～64.8%),11.2%(95%CI,5.5%～17.0%)and 100.0%(95%CI,100.0 0A～100.0%),respectively.Those of combined TCT with PCR-RDB were 100.0%(95%CI,100.0%～100.0%),98.0%(95%CI,96.2%～99.7%),77.3%(95%CI,59.8%～94.8%)and 100.0%(95%CI,100.0%～100.0%),respectively.Conclusion There is good collaboration between TCT and PCR-RDB,and there is high detection valuation when combine TCT with PCR-RDB. |
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| Keywords: | Polymerase Chain Reaction Human papillomavirus 16 Uterine Cervical Neoplasms |
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