菌斑结构的美蓝和革兰氏染色观察

目的：在建立菌斑口内模型的基础上,了解菌斑形成的超微结构。方法：建立一种口内的菌斑模型,将菌斑标本制成半薄切片,分别用革兰氏和美蓝染色后光镜观察。结果：染色显示菌斑中含有球状菌、杆状菌以及丝状菌等细菌,其中部分细菌会出现坏死现象;革兰氏染色显示阳性菌大多为球状或杆状菌,呈散在分布;阴性菌多为丝状或弯曲状菌,分布呈丛状或簇状。结论：建立的菌斑模型较为接近天然菌斑结构,具有一定的应用价值。

Study on the plaque structure in vivo by a dental plaque model

Objective:We established a dental plaque model to observe the structure and formation of plaque. Method :Those specimens were observed by microscope after they were stained by Gram's and Methylene blue. Result: The results by Methylene blue stain showed that the plaque include many bacteria, such as coccus,bacilli,campilobacter and so on, in which some bacteria went to necrosis;The results of plaques stained Gram's showed that positive bacteria were distributed coccus and negative ones were plexiformly distributed campylobacter. Conclusion :The ultrasrtucrure of this dental plaque model was similar to nature's with a certain extent values.