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槲皮黄酮通过Sphk2抑制人皮肤鳞状细胞癌A431细胞的增殖
引用本文:吕君,雷淑英. 槲皮黄酮通过Sphk2抑制人皮肤鳞状细胞癌A431细胞的增殖[J]. 中国麻风皮肤病杂志, 2019, 35(12): 722-726. DOI: 10.12144/zgmfskin201912722
作者姓名:吕君  雷淑英
作者单位:1恩施土家族苗族自治州中心医院皮肤性病诊疗中心,湖北恩施,4450002恩施土家族苗族自治州中心医院皮肤科,湖北恩施,445000
摘    要:目的:研究槲皮黄酮(Quercetin, Qu)对人皮肤鳞状细胞癌A431细胞增殖活性的影响。方法:采用CCK8法、Western blot实验和流式细胞术检测不同浓度下(10、50、100、200 μg/mL)A431细胞活力、增殖及凋亡相关指标;Western blot检测Qu对A431细胞中Sphk2信号通路的影响。结果:10、50、100、200 μg/mL Qu作用96 h后,细胞OD值分别为(0.654±0.098)、(0.527±0.089)、(0.412±0.076)、(0.309±0.054);细胞活力分别为(81.3±4.6)%、(56.3±4.9)%、(37.4±4.2)%、(19.4±3.6)%;细胞凋亡率分别为(6.43±1.09)%、(14.77±1.26)%、(21.30±1.36)%、(29.84±1.26)%。Qu呈浓度依赖性的抑制细胞中Sphk2蛋白表达,且Qu+Sphk2 mimics组细胞活力明显高于于Qu,细胞凋亡率明显低于Qu组(Ps<0.05)。结论:Qu可能通过抑制Sphk2信号通路降低人皮肤鳞状细胞癌细胞的增殖活性。

关 键 词:槲皮黄酮  人皮肤鳞状细胞癌细胞  鞘氨醇激酶2  凋亡  

Quercetin inhibits the proliferation of human skin squamous cell carcinoma A431 cells through sphingosine kinase 2
LYU Jun,LEI Shuying. Quercetin inhibits the proliferation of human skin squamous cell carcinoma A431 cells through sphingosine kinase 2[J]. China Journal of Leprosy and Skin Diseases, 2019, 35(12): 722-726. DOI: 10.12144/zgmfskin201912722
Authors:LYU Jun  LEI Shuying
Affiliation:1. Dermatology and Sexually Transmitted Diseases Clinic, Enshi 445000, China; 2. Department of Dermatology, Central Hospital of Enshi Autonomous Prefecture, Enshi 445000, China
Abstract:Objective: To study the effects of quercetin (Qu) on the proliferative activity of human skin squamous cell carcinoma cells (A431). Methods: The viability, proliferation and apoptosis of A431 cells treated by QU of different concentration (10, 50, 100 and 200 μg/mL) were respectively detected by CCK-8 assay, Western blot and flow cytometry assay. The impacts of Qu on activation of sphingosine kinase 2 (Sphk2) in A431 cells were detected by Western blot. Results: After treated for 96h by QU of concentration 10, 50, 100 and 200 μg/mL, the OD readings were (0.654±0.098),(0.527±0.089),(0.412±0.076)and(0.309±0.054), respectively; the cell activities were (81.3±4.6)%, (56.3±4.9)%, (37.4±4.2)%, (19.4±3.6)% , respectively and the apoptosis rate of A431 cells were (6.43±1.09)%, (14.77±1.26)%,(21.30±1.36)% and (29.84±1.26)% , respectively. Qu dose-dependently inhibited the expression levels of Sphk2 in A431 cells, and the cell activity of A431 cells in Qu+Sphk2 mimics was higher and the apoptosis rate was lower than that in Qu group (Ps<0.05). Conclusion: Qu could inhibit the proliferative activity of human skin squamous cell carcinoma cells through decreasing the activation of Sphk2 pathway.
Keywords:quercetin  human skin squamous cell carcinoma cells  sphingosine kinase 2  apoptosis  
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