不良修复体伴发慢性牙周炎患者外周血清细胞因子分析

目的 探讨不良修复体伴发慢性牙周炎患者体内促炎和抗炎细胞因子的平衡关系及与慢性牙周炎临床评价指标的相关性。方法 采集22例不良修复体伴发慢性牙周炎患者和20例健康对照者外周血，酶联免疫吸附试验（ELISA）检测IL-1β、IL-2、IL-6、TNF-α和IL-10浓度。结果 牙周炎组与正常对照组相比，促炎细胞因子IL-1β、TNF-α显著升高(P＜0.05)，抗炎细胞因子IL-10显著降低(P＜0.05)。牙周炎患者外周血IL-1β与牙周炎临床评价指标正相关，而IL-10与与牙周炎临床评价指标负相关。结论 不良修复体伴发慢性牙周炎患者，抗炎细胞因子与促炎细胞因子的平衡被打破，促炎细胞因子发挥主导作用，参与慢性牙周炎疾病发展。

Study on the role of cytokines in serum of patients with chronic periodontitis accompanied with bad repair body

Objective To evaluate the balance between pro-and anti-inflammatory cytokines in chronic periodontitis patients accompanied with bad repair body and its correlation with different clinical parameters of the periodontal status. Methods Forty two subjects were recruited to the study, including 22 patients with chronic periodontitis(CP) accompanied with bad repair body , 20 healthy controls (HC). Blood samples were collected to assess serum concentrations of IL-1β, IL-2, IL-6, TNF-α and IL-10 by means of enzyme-linked immune-sorbent assay(ELISA). Results Serum levels of pro-inflammatory cytokines (IL-1β and TNF-α) were significantly higher in CP patients than in HC groups (P<0.05), whereas the serum levels of anti-inflammatory cytokines (IL-10) was significantly lower in patients when compared to control (P<0.05). Serum IL-1β level was showed significant positive correlation with each of clinical periodontal parameters. Conversely, serum IL-10 levels had negative significant correlation with each of clinical periodontal parameters. Conclusion Cytokines may play an important role in pro-inflammatory response in serum of patients with chronic periodontitis accompanied with bad repair body. Moreover imbalance between pro- and anti-inflammatory cytokines could be involved in the initiation and progression of chronic periodontitis.