右旋氨基酸对牙龈卟啉单胞菌生物膜形成的影响

目的 本实验研究右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸对牙龈卟啉单胞菌生物膜形成的影响。方法 用含有维生素K(1 μg/mL)和氯化血红素(5 μg/mL)的TSB培养基培养牙龈卟啉单胞菌W83，培养到对数生长期时1：100倍稀释后放入96孔板中，分别加入右旋色氨酸(5 mmol/L)、右旋亮氨酸(8.5 mmol/L)、右旋酪氨酸(3 μmol/L)和右旋蛋氨酸(2 mmol/L)，厌氧培养7 d后结晶紫染色，双蒸水冲洗，干燥后乙醇脱色，紫外分光光度计检测。 结果 加入右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸及空白对照组的吸收值分别为3.70 ± 0.06, 2.74 ± 0.18, 0.71 ± 0.04,3.42 ± 0.02,和3.65 ± 0.01。与空白对照组相比较，加入右旋亮氨酸组吸收值小，并有统计学差异(P < 0.01)。 结论 右旋亮氨酸能够减少牙龈卟啉单胞菌生物膜形成。

The effect of D-animo acid on Porphyromonas gingivalis biofilm formation

Objective To study the effect of D-Tryptophan, D-Leucine, D-tyrosine, and D-Methionine on the formation of biofilm Porphyromonas gingivalis (P. gingivalis) W83. Method: P. gingivalis W83 were cultured in Trypticase soy broth (TSB) supplemented with vitamin K (1μg/ml) and hemin (5 μg/ml) to mid-log phase, and then were diluted 1:100 in 96 well flat-bottom poltstyrene plates and add D-Tryptophan (5mmol/L), D-Leucine (8.5 mmol/L), D-tyrosine (3μmol/L), and D-Methionine (2mmol/L). The biofilms were stained with 25μl of 1% crystal violet, washed with ddH2O and dissolved in ethanol. The absorbance was measured with xMark microplate spectrophotometer at 570nm. Result: The absorbance of P. gingivalis W83 in TSB, D-Tryptophan, D-Leucine, D-tyrosine, and D-Methionine were 3.70 ± 0.06, 2.74 ± 0.18, 0.71 ± 0.04, 3.42 ± 0.02, and 3.65 ± 0.01 respectively. There was significance difference between TSB and D-Leucine groups (p<0.01). Conclusion D-Leucine (8.5 mmol/L) can reduce the biofilm formation of P. gingivalis W83.