LPS对心脏微血管内皮细胞ICAM-1表达的影响及rHDL的调控作用

目的：观察 ＰＬＳ作用下心脏微血管内皮（ＣＭＥＣｓ）细胞间粘附分子 （ＩＣＡＭ）－１的表达情况及ｒＨＤＬ的调控作用，为ＣＨＤ的防治提供实验依据。方 法：利用培养的血管内皮细胞，采用流式细胞仪检测正常细胞、ＬＰＳ作用和ＬＰＳ与 ｒＨＤＬ协同作用下０、１、２、６、１２小时不同时相点 ＩＣＡＭ－１表达阳性细胞数及荧光 强度。结果：ＬＰＳ和ＣＭＥＣｓ共同孵育下，随时间延长ＩＣＡＭ－１表达的阳性细胞数 和荧光强度上升，ｒＨＤＬ有抑制ＩＣＡＭ－１表达作用。结论：ＰＬＳ可以刺激ＣＭＥＣｓ 表达ＩＣＡＭ－１，ｒＨＤＬ对血管内波细胞的损伤有保护作用。

EFFECTS OF LPS AND rHDL ON EXPRESSION OF ICAM-1 IN CARDIAC MICROVASCULAR ENDOTHELIAL CELLS

Objective: To investigate the effects of LPS and recombinant high density lipoprotein(rHDL) on the expression of intercellular adhesion molecule 1(ICAM-1) in cardiac microvascular endothelial cells(CMECs) to provide theoretic basis for prevention and treatment of coronary heart disease(CHD). Methods: After the cardiac microvascular endothelial cells were extracted,they were randomized into three groups(A,B and C). The cells in group A were routinely cultured while those in groups B and C were respectively cultured in the media with addition of LPS and rHDL. Then the number of cells with positive expression of ICAM-1 and florescent density of the expression were determined in 0,1,2,6 and 12h after incubation with flow cytometry. Results: After the CMECs were incubated in the medium with addition of LPS,the number of the cells with positive expression of ICAM-1 and florescent density of the expression were gradually increased with time prolongation. And rHDL could inhibit the expression of ICAM-1. Condclusion: LPS can stimulate CMECs to express ICAM-1 and rHDL might protect the vasular endothelial cells from damage.