| IKCa1通道阻滞剂TRAM-34对HUVEC增殖作用的影响 |
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| 引用本文： | 郭洪宇,张雅芳,林芳,遇春林,杨慧科,李晓冬.IKCa1通道阻滞剂TRAM-34对HUVEC增殖作用的影响[J].解剖学研究,2011,33(6):417-421. |
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| 作者姓名： | 郭洪宇 张雅芳 林芳 遇春林 杨慧科 李晓冬 |
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| 作者单位： | 哈尔滨医科大学基础医学院人体解剖学教研室,黑龙江 哈尔滨,150081 |
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| 摘 要： | 目的 体外应用IKCa1通道阻滞剂TRAM-34干预人脐静脉内皮细胞(HUVEC),探讨其抑制HUVEC增殖作用的影响.方法 采用免疫荧光和RT-PCR法观察HUVEC细胞IKCa1的表达,常规MTT法分析不同浓度TRAM-34作用24、48、72 h后HUVEC细胞增殖的变化.结果 免疫荧光和RT-PCR法均证实HU...
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| 关 键 词： | IKCa1通遭 TRAM-34 人脐静脉内皮细胞 |
Effects of the IKCa1 channel inhibitor TRAM-34 on proliferation of human umbilical vein endothelial cells |
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| GUO Hong-yu
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ZHANG Ya-fang
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LIN Fang
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YU Chun-lin
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YANG Hui-ke
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LI Xiao-dong.Effects of the IKCa1 channel inhibitor TRAM-34 on proliferation of human umbilical vein endothelial cells[J].Anatomy Research,2011,33(6):417-421. |
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| Authors: | GUO Hong-yu ZHANG Ya-fang LIN Fang YU Chun-lin YANG Hui-ke LI Xiao-dong |
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| Abstract: | Objective To examine the effects of the IKCa1 channel inhibitor TRAM-34 on proliferation of human umbilical vein endothelial cells(HUVEC) in vitro.Methods Double-immunofluorescence staining and RT-PCR experiments were done to evaluate the expressions of IKCa1 in HUVECs.In this present study,the effects of TRAM-34 on HUVECs proliferation were investigated by MTT assay with varying concentrations of TRAM-34 treatment for 24 h,48 h and 72 h.Results Using the HUVEC,the normal expression of IKCa1 in HUVECs was confirmed,and found that TRAM-34(>10 μmol) treatment for over 24 h suppressed remarkably HUVECs,exerting a significant cytotoxic effect upon HUVECs in a dose-dependent(0-30 μmol) and time-dependent(24-72 h) manner.Conclusions It is suggested that HUVEC expressed IKca1,and TRAM-34 treatment resulted in a significant dose-dependent and time-dependent inhibition in the growth of HUVEC. |
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| Keywords: | IKCa1 channel TRAM-34 Human umbilical veicn endothelial cells(HUVEC) |
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