| SDF-1/CXCR4生物轴活化诱导对鼠骨髓源性内皮祖细胞生物学性能的影响 |
| |
| 引用本文： | 康庆林,陆联松,陈佳. SDF-1/CXCR4生物轴活化诱导对鼠骨髓源性内皮祖细胞生物学性能的影响[J]. 解剖学研究, 2011, 33(6): 413-416 |
| |
| 作者姓名： | 康庆林 陆联松 陈佳 |
| |
| 作者单位： | 上海交通大学附属第六人民医院骨科 上海市第六人民医院骨科,上海,200233 |
| |
| 基金项目： | 上海市卫生局面上基金,上海交通大学医工交叉面上基金 |
| |
| 摘 要： | 目的 观察基质细胞衍生因子-1( SDF-1)及其特异性受体CXCR4生物轴对体外培养的鼠骨髓源性内皮祖细胞(EPCs)生物学性能的影响.方法 以密度梯度离心法获取大鼠骨髓单个核细胞,由Dil-ac-LDL和FITC-UEA-1荧光双染鉴定后确认为EPCs.传代培养后,加入不同浓度SDF-1溶液,培养48 h,然后分别...
|
| 关 键 词： | 基质细胞衍生因子-1 内皮细胞 细胞增殖 SDF-1/CXCR4轴 血管吻合 |
Effects of SDF-1/CXCR4 axis on biological characteristics of bone marrow derived endothelial progenitor cells in rat in vitro |
| |
| KANG Qing-lin , LU Lian-song , CHEN Jia. Effects of SDF-1/CXCR4 axis on biological characteristics of bone marrow derived endothelial progenitor cells in rat in vitro[J]. Anatomy Research, 2011, 33(6): 413-416 |
| |
| Authors: | KANG Qing-lin LU Lian-song CHEN Jia |
| |
| Abstract: | Objective To explore the effects of SDF-1 on endothelial progenitor cells(EPCs).Methods The mono nuclear cells were isolated from bone marrow of rats by density gradient centrifugation combined with adherence cells filtration,then the isolated cells were plated on fibronectin coated culture dishes.After 7 days,adherent cells were kept with different concentrations of SDF-1 for 48 hours.EPCs proliferation was determined by MTT test;EPCs migration ability assay was performed by Transwell test;The CXCR4 antibody expression in cultured EPCs was examined by IHC.Results SDF-1 increased the number of EPCs in the dose-depending manner(P<0.01or <0.05) : SDF-1 promoted EPCs proliferation and migration capacity(P<0.01),EPCs presented CXCR4 positive expression after induction by SDF-1 in dose-depending manner.Conclusion SDF-1/CXCR4 biological axis can increase the number of bone mar row derived EPCs and improve their biological characteristics. |
| |
| Keywords: | Stroma cell derivated factor(SDF)-1 Endothelial progenitor cells Cell proliferation SDF-1/CXCR4 biological axis Vessel anamostosis |
| 本文献已被 万方数据 等数据库收录！ |
