| 乙肝病毒表面抗原基因疫苗诱导小鼠细胞及体液免疫应答的 … |
| |
| 引用本文： | 刘双虎,燕美玉.乙肝病毒表面抗原基因疫苗诱导小鼠细胞及体液免疫应答的 …[J].湖南医科大学学报,1999,24(4):313-315. |
| |
| 作者姓名： | 刘双虎 燕美玉 |
| |
| 作者单位： | [1]湖南医科大学传染病研究所 [2]湖南医科大学免疫研究室 |
| |
| 摘 要： | 为观察乙肝病毒ＨＢｓＡｇ基因疫苗免疫小鼠后的细胞及体液免疫应答，将该疫苗定向克隆于质粒ｐｅＤＮＡ３巨细胞病毒启动子下游，构建能在真核细胞内表达的重组基因疫苗ｐｃＤＮＡ－ＨＢｓＡｇ。将此疫苗通过多点肌肉注射免疫Ｂａｌｂ／Ｃ小鼠后第六周，实验组小鼠均出现抗－ＨＢｓＩｇＧ阳性，其抗体水平明显高于对照组，同时Ｔｈ１免疫应答相关的细胞因子ＩＬ－２及γ干扰素水平显高于对照组，表明本文构建的基因疫苗ｐｃＤＮＡ３
|
| 关 键 词： | 乙型肝炎病毒 基因疫苗 DAN 小鼠 免疫应答 |
Specific cellular and humoral immune responses induced by intramuscular injection of DNA vaccine containing HBV HBsAg gene in mice] |
| |
| S Liu,D Tan,C Li.Specific cellular and humoral immune responses induced by intramuscular injection of DNA vaccine containing HBV HBsAg gene in mice][J].Bulletin of Hunan Medical University,1999,24(4):313-315. |
| |
| Authors: | S Liu D Tan C Li |
| |
| Institution: | Institute of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha 410008. |
| |
| Abstract: | To know the influences on the function of cellular and humoral immunity in mice by DNA vaccine containing HBV HBsAg gene, the DNA vaccine, pcDNA3-HBsAg, in which the HBsAg gene sequences of HBV were inserted into downstream of CMV promoter in pcDNA3, were injected intramuscularly into Balb/C mice. The results were that the serum anti-HBsIgG was detected in all of the pcDNA3-HBsAg immunized mice, but none in control mice; serum levels of Th1 cytokines, interleukine 2, and interferon gamma, were significantly higher in pcDNA3-HBsAg immunized mice than these in controls. It is indicated that the good responses of cellular and humoral immunity can be induced by injection of pcDNA3-HBsAg in Balb/C mice. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
|
