首页 | 本学科首页   官方微博 | 高级检索  
     

高效液相色谱法测定嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱含量
引用本文:王岩,刘欣欣. 高效液相色谱法测定嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱含量[J]. 中国药业, 2011, 20(17): 33-35
作者姓名:王岩  刘欣欣
作者单位:1. 沈阳药科大学药学院,辽宁,沈阳,110016
2. 辽宁普诚医药有限公司,辽宁,沈阳,110014
摘    要:目的建立测定嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱含量的高效液相色谱法。方法色谱柱为Symmetry C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水-氯仿-冰醋酸-三乙胺(50∶50∶2∶1∶0.6),柱温为25℃,流速为1.0 mL/min,检测波长为254 nm。结果乌头碱、中乌头碱和次乌头碱的质量浓度均在9.0~45.0μg/mL范围内与峰面积线性关系良好,回归方程分别为A1=2.125×106C+1.252×104,A2=2.143×106C+1.422×104,A3=1.968×106C-2.152×104,相关系数r分别为0.999 6,0.999 8,0.999 8,平均回收率分别为100.40%,99.61%,100.15%,RSD分别为0.72%,0.86%,0.89%。结论所用方法简便、快速、准确,适用于嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱的含量测定。

关 键 词:高效液相色谱法  嘎日迪五味丸  乌头碱  中乌头碱  次乌头碱  含量测定

Content Determination of Aconitine,Mesaconitine and Hypaconitine in Garidi-5 Pills by HPLC
Wang Yan,Liu Xinxin. Content Determination of Aconitine,Mesaconitine and Hypaconitine in Garidi-5 Pills by HPLC[J]. China Pharmaceuticals, 2011, 20(17): 33-35
Authors:Wang Yan  Liu Xinxin
Affiliation:Wang Yan1,Liu Xinxin2(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang,Liaoning,China 110016,2.Liaoning Pucheng Pharmaceutical Co.,Ltd.,China 110014)
Abstract:Objective To establish HPLC method for determining the content of aconitine,mesaconitine and hypaconitine in Garidi-5 Pills.Methods The chromatographic column was Symmetry C18(150 mm×4.6 mm,5 μm),the mobile phase was methol-water-chloroform-glacial acetic acid-thriethylamine(50:50:2:1:0.6),the column temperature was 25 ℃,the flow rate of 1.0 mL/min,the UV detection wavelength was 254 nm.Results The linear range of concentration of three compounds was 9.0-45.0 μg/mL.Regression equations were A1=2.125×106C+1.252×104,A2=2.143×106C+1.422×104,A3=1.968×106C-2.152×104,r were 0.999 6,0.999 8,0.999 9,respectively.The average recoveries and the relative standard deviation were 100.40%,99.61%,100.15%and 0.72%,0.86%,0.89%,respectively.Conclusion The method is simple,fast and accurate.It is suitable for content determination of aconitine,mesaconitine and hypaconitine in Garidi-5 Pills.
Keywords:HPLC  Garidi-5 Pills  aconitine  mesaconitine  hypaconitine  content determination  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号