| 高效液相色谱法测定脑力静糖浆中甘草次酸含量 |
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| 引用本文： | 孟建升,蒋俊春,何波. 高效液相色谱法测定脑力静糖浆中甘草次酸含量[J]. 中国药业, 2011, 20(16): 40-41 |
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| 作者姓名： | 孟建升 蒋俊春 何波 |
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| 作者单位： | 河南省商丘市食品药品检验所,河南,商丘,476000 |
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| 摘 要： | 目的建立测定脑力静糖浆中甘草次酸含量的高效液相色谱法。方法色谱柱为Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-1%冰醋酸(80∶20),流速1.0 mL/min,检测波长250 nm。结果甘草次酸质量浓度在13.44～134.4μg/mL范围内与峰面积积分值线性关系良好,线性回归方程为Y=37 856.66 X+8.63(r=0.999 98),平均回收率为99.06%,RSD=0.62%(n=5)。结论该方法操作简便、专属性强、结果准确,可用于脑力静糖浆的质量控制。
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| 关 键 词： | 脑力静糖浆 高效液相色谱法 含量测定 甘草次酸 |
Determination of Glycyrrhetinic Acid in Naolijing Syrup by HPLC |
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| Meng Jiansheng,Jiang Junchun,He Bo. Determination of Glycyrrhetinic Acid in Naolijing Syrup by HPLC[J]. China Pharmaceuticals, 2011, 20(16): 40-41 |
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| Authors: | Meng Jiansheng Jiang Junchun He Bo |
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| Affiliation: | Meng Jiansheng,Jiang Junchun,He Bo(Shangqiu Institute for Drug Control,Shangqiu,Henan,China 476000) |
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| Abstract: | Objective To establish a HPLC method for the determination of glycyrrhetinic acid in Naolijing Syrup.Methods The chromatographic system consisted of Agilent Eclipse XDB-C18 column（250 mm×4.6 mm,5 μm） with methanol-1%glacial acetic acid（80 ∶20） as the mobile phase.The flow rate was 1.0 mL/min and the detecting wavelength was 250 nm.Results Glycyrrhetinic acid had the good linear relation in the range of 13.44-134.4 μg/mL.The linear equation was Y=37 856.66X＋8.63（r=0.999 98）,the average recovery rate was 99.06%,RSD=0.62%（n=5）.Conclusion The established method is simple,convenient and accuracy with strong specificity,which can be used for the quality control of Naolijing Syrup. |
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| Keywords: | Naolijing syrup HPLC content determination glycyrrhetinic acid |
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