藏药材印度獐牙菜质量标准研究

 目的 建立印度獐牙菜药材的质量标准。方法 采用薄层色谱法鉴别印度獐牙菜药材中獐牙菜苦苷、龙胆苦苷和芒果苷成分；以高效液相色谱法测定该3种成分的含量。色谱柱为Zorbax Eclipse XDB-C₁₈（4.6 mm×250 mm，5 μm），流动相为甲醇-水（0.1%冰醋酸）线性梯度洗脱，甲醇体积分数从22%经18 min至32% 并保持5 min，流速为1.0 mL·min-1，柱温为25 ℃，检测波长为243 nm。结果 印度獐牙菜药材中獐牙菜苦苷、龙胆苦苷和芒果苷的薄层色谱鉴别特征明显，专属性强；药材中獐牙菜苦苷、龙胆苦苷和芒果苷的含量测定线性范围分别为0.06~2.00 μg(r=0.999 7)，0.09~2.90 μg(r=0.999 7)和0.05~1.65 μg(r=0.999 8)，平均回收率（n=9）分别为103.76 % (RSD=1.34 %)，99.43 % (RSD=1.60%)和96.22% (RSD=1.30%)。结论 所建立的印度獐牙菜定性定量测定方法简单准确，能够为印度獐牙菜药材的质量控制提供有效数据。

Quality Control of Traditional Tibetan Medicine Swertia chirayita

OBJECTIVE To establish a method of quality control of Swertia chirayita （Roxb.ex Fleming） Karsten. METHODS Swertiamain, gentiopicrin and mangiferin was identified by TLC in Swertia chirayita （Roxb.ex Fleming） Karsten. Its content was determined by HPLC equipped with, Zorbax Eclipse XDB-C₁₈（4.6 mm×250 mm,5 μm）column. The mobile phase consisted of methanol-water(0.1% glacial acetic acid), which concentration of methanol from 22% to 32% in 18 min, then kept for 5 min. The anlysis was done at a flow rate of 1.0 mL·min-1. The column temperature was 25 ℃ and the detection wavelength was 243 nm. RESULTS The linearities of swertiamain, gentiopicrin and mangiferin were in the ranges of 0.06-2.00 μg(r=0.999 7)，0.09-2.90 μg (r=0.999 7) and 0.05-1.65 μg(r=0.999 8), respectively. The average recoveries (n=9) were 103.76% (RSD=1.34%) for swertiamain，99.43% (RSD=1.60%) for gentiopicrin and 96.22% (RSD=1.30%) for mangiferin. CONCLUSION This method is simple, accurate with good reproducibility. It is suitable for quality control of Swertia chirayita（Roxb.ex Fleming） Karsten.