兔子宫血供阻断后的病理研究

目的：研究新西兰兔子动脉阻断后子宫组织、雌、孕激素受体（ER、PR）表达的变化，以及侧支循环建立的情况，为临床研究提供实验基础。材料和方法：15只新西兰大白兔，通过显微手术阻断子宫动脉血流，模拟UAE术后的病理改变，分两组观察实验兔子宫组织变化情况，侧支循环建立情况，并采用免疫组化技术（LSAB法）对术前后雌、孕激素受体表达变化进行半定量分析。结果：15只新西兰兔在术后30d建立了不同程度的侧支循环，HE染色切片观察未见到明显的平滑肌细胞变性坏死。对两组激素受体的实验数据统计分析表明，第一组术前与术后30d比较ER、PR表达改变无统计学意义，但其术前、术后3d、术后15d、术后30d ER、PR动态变化具有统计学意义，术后饲养一段时间，ER、PR表达出现上升趋势。第二组术前与术后30d比较ER、PR改变有显著差异。手术方法的不同对激素受体的表达具有一定的影响。结论：阻断新西兰大白兔宫血流后饲养一段时间，将会建立侧支循环，ER、PR表达经过一段时间的下降，又出现上升趋势，这是否提示UAE术长期疗效不佳，有待于进一步研究。

Pathological Study after Interruption of Blood Supply to Rabbit Uterine

Purpose: To investigate the changes of the estrogen and progesterone receptors expression of the uterine tissues after blocking the uterine artery and the establishment of the compensatory circulation for providing the basis of the clinical trials.Materials and Methods: The uterine artery of 15 female New Zealand white rabbits were interrupted by microsurgery in order to imitate the pathological changes of UAE. Two groups of rabbits were divided for observing the changes of uterine tissues, the establishment of compensatory circulation.The expressions of ER, PR changes were analyzed by using immunohisto-chemistry(LSAB) . Results: The compensatory circulation of all female New Zealand white rabbits were established at different degrees in 30d postoperation. The degeneration and necrosis of the tissues were not observed in HE slides. There was no statistically significance of ER, PR expressions between pre -operation and on the 30th day postoperation in the 1st group. The dynamistic changes were observed as the ER, PR expression compared at preoperation, 3rd day, 15th day, 30th day postoperation. The expressions of ER, PR had a tendency to increase when it took a short time postoperation. There was significant difference between pre - operation and 30th day postoperation in the 2nd group. The difference of operation had significant affect on the expression of ER, PR between two groups.Conclusion: There will be established the compensatory circulation when it took a short time after the interruption of the uterine artery. The expressions of the ER, PR decrease at first and then increase after a short time. If it meaned that the long - term effectiveness of UAE will be poor, further study would be necessary.