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| Screening and Identification of a Novel Hepatocellular Carcinoma Cell Binding Peptide by Using a Phage Display Library | |
| 作者姓名： | 朱小华 吴华 罗莎 鲜于志群 祝丹 |
| 作者单位： | Department of Nuclear Medicine Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Nuclear Medicine，Xiamen First Hospital，Fujian Medical University，Department of Nuclear Medicine，Tongren Hospital，Department of Nuclear Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Nuclear Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Xiamen 361003，China，Beijing 100730，China，Wuhan 430030，China，Wuhan 430030，China |
| 摘 要： | The purpose of this study was to screen peptides that can specifically bind to human hepatocellular carcinoma(hHCC) cells using phage display of random peptide library in order to de-velope a peptide-based carrier for the diagnosis or therapy of hHCC.A peptide 12-mer phage display library was employed and 4 rounds of subtractive panning were performed using the hHCC cell line HepG2 as the target.After panning,the phages that specifically bound to and internalized in hHCC cells were selected.The selected phages demonstrated highly specific affinity to HepG2 cells analyzed by ELISA and immunofluorescence analysis.57.3% of the selected phage clones displayed repeated sequence FLLEPHLMDTSM,and 4 amino acid residues,FLEP were extremely conservative.Based on the sequencing results,a 16-mer peptide(WH-16) was synthesized.The competitive ELISA showed that the binding of the phage clones displayed sequence FLLEPHLMDTSM to HepG2 cells was efficiently inhibited by WH-16.Our findings indicate that cellular binding of phage is mediated via its displayed peptide and the synthesized 16-mer peptide may have the potential to be a delivery carrier in target diagnosis or therapy for hHCC. |
| 关 键 词： | 肝细胞癌 肽 抗菌素 生物靶点 |
Screening and identification of a novel hepatocellular carcinoma cell binding peptide by using a phage display library |
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| Xiaohua?Zhu,Hua?Wu,Sha?Luo,Zhiqun?Xianyu,Dan?Zhu.Screening and Identification of a Novel Hepatocellular Carcinoma Cell Binding Peptide by Using a Phage Display Library[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(3):299-303. | |
| Authors: | Xiaohua Zhu Hua Wu Sha Luo Zhiqun Xianyu Dan Zhu |
| Institution: | 1. Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2. Department of Nuclear Medicine, Xiamen First Hospital, Fujian Medical University, Xiamen 361003, China 3. Department of Nuclear Medicine, Tongren Hospital, Beijing 100730, China |
| Abstract: | The purpose of this study was to screen peptides that can specifically bind to human hepatocellular carcinoma(hHCC) cells using phage display of random peptide library in order to de-velope a peptide-based carrier for the diagnosis or therapy of hHCC.A peptide 12-mer phage display library was employed and 4 rounds of subtractive panning were performed using the hHCC cell line HepG2 as the target.After panning,the phages that specifically bound to and internalized in hHCC cells were selected.The selected phages demonstrated highly specific affinity to HepG2 cells analyzed by ELISA and immunofluorescence analysis.57.3% of the selected phage clones displayed repeated sequence FLLEPHLMDTSM,and 4 amino acid residues,FLEP were extremely conservative.Based on the sequencing results,a 16-mer peptide(WH-16) was synthesized.The competitive ELISA showed that the binding of the phage clones displayed sequence FLLEPHLMDTSM to HepG2 cells was efficiently inhibited by WH-16.Our findings indicate that cellular binding of phage is mediated via its displayed peptide and the synthesized 16-mer peptide may have the potential to be a delivery carrier in target diagnosis or therapy for hHCC. |
| Keywords: | phage display of random peptide library hepatocellular carcinoma peptides biologictargeting delivery |
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