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| 高效液相色谱法测定颈康胶囊中葛根有效成分葛根素的含量 | |
| 引用本文： | 彭兆斌,范艳冰,汤婉芬.高效液相色谱法测定颈康胶囊中葛根有效成分葛根素的含量[J].世界中西医结合杂志,2009,4(7):480-482. |
| 作者姓名： | 彭兆斌 范艳冰 汤婉芬 |
| 作者单位： | 1. 广州市荔湾区第三人民医院,广东,广州,510380 2. 广州医学院羊城医院,广东,广州,510140 3. 广州固志医药科技有限公司,广东,广州,510663 |
| 摘 要： | 目的建立高效液相色谱法（HPLC）测定中成药颈康胶囊中葛根的主要成分葛根素的含量测定方法。方法采用Betasil（4.6mm×250mm,5μm）色谱柱;检测波长：250nm;流动相：甲醇-水（25∶75）;流速：1.0mL/min。结果葛根素在0.14～0.694μg范围内具有良好的线性关系,相关系数r=0.999853,平均回收率为102.51%,RSD=1.24%。结论该方法灵敏度高、专属性强、重现性好,并通过对数批样品含量进行考察,可作为制剂质量控制指标之一。 |
| 关 键 词： | 颈康胶囊 HPLC 葛根 葛根素 |
Determination of Effective Ingredient Puerarin with HPLC in Jing Kang Jiaonang |
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| PENG Zhao-bin,FAN Yan-bing,TANG Wan-fen.Determination of Effective Ingredient Puerarin with HPLC in Jing Kang Jiaonang[J].World Journal Of Integrated Traditional and Wesrern Medicine,2009,4(7):480-482. | |
| Authors: | PENG Zhao-bin FAN Yan-bing TANG Wan-fen |
| Institution: | PENG Zhao -bin, FAN Yan -bing, TANG Wan -fen (1. Guangzhou Liwan District No. 3 People＇s Hospital, Guangzhou Guangdong 510380;2. Yangeheng Hospital Affiliated to Guangzhou Medical College, Guangzhou Guangdong 510140;3. Guangzhou Guzhi Medical Sci- ence and Technology Co. Ltd. Guangzhou Guangdong 510663) |
| Abstract: | Objective To establish the method of determination of puerarin content,the main ingredient of Chinese herbal patent,Jing Kang Jiaonang with high performance liquid chromatography(HPLC).Methods Betasil chromatographic column(4.6 mm×250 mm,5 μm)was adopted,the detective rate was 1 mL/min.Results Good linear relation of puerarin was obtained in the range of 0.14 μg to 0.694 μg.Correlation coefficient r=0.999 853,the average recovery rate was 102.51%,and RSD=1.24%.Conclusion The method is high in sensitivity,strong in specialty and good in reproducibility.By the observation of puerarin contents of several batches of samples,it figures out that this method can be taken as one of the indexes to evaluate the quality of the preparation. |
| Keywords: | HPLC Jing Kang Jiaonang HPLC Radis Puerariae Puerarin |
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