| 阿普唑仑血药浓度的RP-HPLC测定方法 |
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| 引用本文： | 费洪荣,曲晓兰,高允生,齐永秀.阿普唑仑血药浓度的RP-HPLC测定方法[J].泰山医学院学报,2004,25(6):646-648. |
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| 作者姓名： | 费洪荣 曲晓兰 高允生 齐永秀 |
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| 作者单位： | 泰山医学院药学院,山东,泰安,271000 |
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| 摘 要： | 目的建立阿普唑仑血药浓度的反相HPLC测定方法.方法固定相为ODS 色谱柱(粒径5 μm,150×3.9 mm ID);甲醇-0.02M pH=5.0的醋酸铵(60∶40)为流动相;流速为1.0 ml/min;检测波长为223 nm(阿普唑仑的最大吸收波长).结果本法最低检出浓度为5 ng/ml,线性范围为0.01～50 μg/ml,平均回收率为98.67%,日内和日间平均变异系数分别为2.44%和2.74%.结论用HPLC法测定血清中ALP的含量既简便又可行,可作为阿普唑仑血药浓度的测定方法.
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| 关 键 词： | 阿普唑仑 反相高效液相色谱 含量测定 |
| 文章编号： | 1004-7115(2004)-0646-03 |
| 修稿时间： | 2004年8月25日 |
RP HPLC determination of alprazolam in serum |
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| FEI Hong-rong,QU Xiao-lan,GAO Yun-sheng,QI Yong-xiu.RP HPLC determination of alprazolam in serum[J].Journal of Taishan Medical College,2004,25(6):646-648. |
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| Authors: | FEI Hong-rong QU Xiao-lan GAO Yun-sheng QI Yong-xiu |
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| Abstract: | Objective: To determine the alprazolam in serum by RP-HPLC. Methods: Chromatographic conditions were adopted with ODS Column as solid phase, Methanol:NH4Ac=60:40 as mobile phase, the flow rate of mobile phrase at 1.0 ml/min and alprazolam detection at 223nm. Results: The average recoveries of alprazolam were 98.67%(n=5). RSD with in-day was 2.44% and RSD between-day was 2.74%. Conclusion: The method is simple, fast and reliable, and can be used for the determination of ALP in serum. |
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| Keywords: | alprazolam RP-HPLC assaying |
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