P53基因突变与口腔鳞癌关系的研究

本文采用多聚酶链反应技术及单链构象多态分析（ＰＣＲ／ＳＳＣＰ）检测了３４例口腔鳞癌及２０例癌旁组织中Ｐ５３基因Ⅴ、Ⅷ外显子，发现癌组织中２０例出现基因突变，阳性率为５８．８％，而在癌旁组织中无１例出现突变，本研究表明，口腔鳞癌的产生与Ｐ５３突变有关。

CORRELATION OF MUTATIONS OF P 53 GENE WITH ORAL MUCOUS SQUAMOUS CELL CARCINOMA

Exons Ⅴ Ⅷ of the P 53 gene in 34 cases of oral mucous squamous cell carcinoma and 22 cases of tissues immediately adjacent to carcinoma were studied using polymerase chaine reaction-single strand conformation polymorphism andysis (PCR-SSCP).The incidence of P 53 mutations was 58.8%(20/34)in oral mucous squamous cell carcinoma, and no mutation was discovered in all case of tissues immdiately adjacent to carcinomas. The mutations were more frequently found in the poor differentiative carcinoma than that in the higher differentiative ones, although no statistical signifience was noticed. On the other hand,we also analysed the corretation of the smoking history with the incidence of P 53 gene mutation,18 out of 24 smokers had P 53 gene mutation whereas 2 out of 10 non-smokers in contrast were found to have P 53 gene mutation (P=0.0184). The results suggest that the carcinogenesis of oral squamous cell carcinoma may be retated to the mutatons of P 53 gene and smoking may stimulate the mutation of P 53 gene, perhaps it play an important role in the carcinogenesis of oral mucous. gene