| 一种较好的RNA分离制备方法及其在人胚RNA提取中的应用 |
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| 引用本文： | 谢慎思,袁恬莹,陈汉春,杨友云,傅敏庄.一种较好的RNA分离制备方法及其在人胚RNA提取中的应用[J].中南大学学报(医学版),1986(3). |
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| 作者姓名： | 谢慎思 袁恬莹 陈汉春 杨友云 傅敏庄 |
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| 作者单位： | 湖南医学院分子生物学研究室
(谢慎思，袁恬莹，陈汉春，杨友云)，湖南医学院分子生物学研究室(傅敏庄) |
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| 摘 要： | 以钒基核苷酸复合物为核酸酶抑制剂,从人胚组织中制备总RNA,所得产品几乎不含DNA和蛋白质,经过分离Poly(A)~ mRNA,证明制品有合成cDNA的完整功能,方法比较简捷,适于大量制备以满足分离mRNA 之需,并讨论了此法的优缺点。
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| 关 键 词： | 核糖核酸 脱氧核糖核酸 核糖核酸 信使 转录遗传 基因 胚胎 人类 分离与提纯 方法 |
A RECOMMENDABLE METHOD FOR RNA ISOLATION AND IT S APPLICATION TO HUMAN EMBRYONIC TISSUE RNA EXTRACTION |
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| Xie Shensi Yuan Tianying Chen Hanchun Yang Youyun Fu MinzhuangLaboratory of Molecular Biology,Hunan Medical college.A RECOMMENDABLE METHOD FOR RNA ISOLATION AND IT S APPLICATION TO HUMAN EMBRYONIC TISSUE RNA EXTRACTION[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1986(3). |
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| Authors: | Xie Shensi Yuan Tianying Chen Hanchun Yang Youyun Fu MinzhuangLaboratory of Molecular Biology Hunan Medical college |
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| Abstract: | The preparntion of human embryonic tissues total RNA of high quality was carried out by using vanadylribonucleoside complexes (VRC) as RNAase inhibitor in addttion to phenol and chloroform extraction. The products were almost free from DNA and protein, only with occasional trace ammount of DNA. After poly (A) RNA selection, it was proved to be functionally intact in cDNA synthesis. The method is comparatively simple and efficient. It can be used in relatively large quantity of RNA preparatjon and thus for further mRNA isolation as well. The advantages and limitations of this method were discussed. |
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