胆红素脑病仔兔脑组织氨基酸含量的研究

目的 探讨胆红素脑病仔兔脑组织中氨基酸含理的变化。方法 采用生后3-5天纯系仔兔腹腔注射胆红素200mg/kg方法制备胆红素脑病动物模型。应用日立835-50型氨基酸分析仪检测脑组织的氨基酸含量。结果 胆红素脑病脑组织中谷氨酸，门冬氨酸含量下降；γ-氨基丁酸，丙氨酸，酪氨酸、缬氨酸，苯丙氨酸升高。结论 脑组织中递质性氨基酸含量的变化提示兴奋性氨基酸的兴奋性毒性作用可能导致仔兔胆红素脑病的发生；非递质性氨基酸水平的升高证实了胆红素可抑制脑组织蛋白质的生物合成。

Initial study on the inhibitor of thioredoxin reductase in earthworm

ObjectiveTo study change of aminoacid content in the brain of newborn rabbits with bilirubin encephalopathy. MethodsNewborn rabbits (3-5 days after birth) with bilirubin encephalopathy were induced by administration intra peritoneally with bilirubin (200mg/kg). Aminoacid content in brain were analysed by Hitachi 835 50 type aminoacid analysis instrument.ResultsThe content of glutamate and aspartate in brain tissue with bilirubin encephopathy decrease; GABA, alanine, tyrosine, valine and pheny lalanine increase. ConclusionsThe change of content of neurotransmitters in brain indicates that excitotoxicity of EAAs may contribute to the bilirubin encephalopathy. The increase of non neurotransmitters level proves that bilirubin may inhibit the biologic composition of protein in brain.