miR-141过表达增强人食管癌细胞的放射敏感性

目的 研究miR-141对食管癌细胞放射敏感性的影响及可能的作用机制。方法 将miR-141 mimic或miR-对照转染到食管癌KYSE-150细胞中，分别使用qRT-PCR和Western blot技术检测miR-141和增殖相关蛋白Ki67、凋亡相关蛋白Bax和Bcl-2的表达。CCK-8，流式细胞术及克隆形成实验检测放射处理后细胞的放射敏感性变化。结果 随放射剂量的增加，食管癌细胞中miR-141表达逐渐降低（t=2.57~8.96，P<0.05）。miR-141过表达抑制了细胞的增殖和克隆形成能力，促进了KYSE-150细胞凋亡，使得放射敏感性增高（t=3.24，P<0.05）。此外，与对照组相比，miR-141过表达显著抑制KYSE-150细胞中的Ki67和Bcl-2蛋白表达（t=6.56、8.24，P<0.01），并促进Bax蛋白表达（t=3.24，P<0.01），进一步证实了miR-141增强食管癌细胞的放射敏感性。结论 miR-141通过调控细胞增殖和凋亡相关蛋白表达增强食管癌细胞的放射敏感性。

Over-expression of miR-141 increases the radiosensitivity of esophagus cancer cells

Objective To investigate the role and potential molecular mechanism of the radiosensitivity of esophagus cancer cells. Methods miR-141 mimics or its negative control was transfected into esophagus cancercells KYSE-150, respectively. Radiosensitivity of esophagus cancer cells was determined by CCK-8, flow cytometry and colony formation assay. The expression level of miR-141 was determined by qRT-PCR. Western blot was used to detect the expressions of proliferation-related protein Ki67 and apoptosis-related proteins Bax and Bcl-2. Results After radiation, the expression of miR-141 was decreased in KYSE-150 cells in a dose-dependent manner (t=2.57-8.96, P<0.05). Up-regulation of miR-141 significantly suppressed cell proliferation and colony formation and promoted apoptosis, indicating that overexpression of miR-141 enhanced the radiosensitivity of KYSE-150 cells(t=3.24, P<0.05). In addition, the miR-141 mimic significantly reduced the expressions of Ki67 and Bcl-2 protein (t=6.56, 8.24, P<0.01) and inhibited the expression of Bax protein compared with miR-control group (t=3.24, P<0.01). Conclusions Over-expression of miR-141 enhances the radiosensitivity of esophagus cancer cells by regulating the expressions of proliferation-related protein Ki67 and apoptosis-related proteins Bax and Bcl-2.