实验性干眼大鼠的角结膜上皮组织学改变

目的建立大鼠干眼模型并进行评价,比较正常大鼠和干眼大鼠角结膜上皮组织形态学特点。方法采用苯扎氯铵滴眼诱导大鼠出现干眼,麻醉后处死动物,取正常大鼠和干眼大鼠角结膜组织行HE染色,及电镜下观察大鼠角结膜超微结构。结果干眼大鼠角膜上皮和结膜上皮出现上皮细胞缺失,结膜上皮杯状细胞减少,电镜下观察干眼大鼠角膜结膜上皮细胞微绒毛缺失,断裂,出现基底膜溶解,细胞内线粒体溶解。结论苯扎氯铵诱导的大鼠干眼模型与人干眼发生后角膜结膜发生的改变相似,此干眼模型在动物实验研究中具有重要的参考价值。

Morphological changes of corneal and conjunctival epithelia in a rat model of dry eye

Objective To evaluate the rat model of dry eye induced by topical administration of benzalkonium chloride and compare the morphological characteristics of corneal and conjunctival tissues of the normal and model rats. Methods Topical administration of benzalkonium chloride (0.3%) twice per day for seven days to induce the rat model of dry eye. Pathology with HE and periodic acid-Schiff staining and transmission electron microscopy were performed to evaluate the corneal and conjunctival changes. Results The corneal and conjunctival epithelial cells were sloughed off, the number of goblet cells was decreased in the dry eye model. Microvilli were obviously lost in the surface epithelial cells of the dry eye rat cornea and conjunctiva compared with the normal rats, as indicated by the amount and size of microvilli. Conclusions The changes in the corneal and conjunctival epithelia of BAC-induced rat dry eye are similar to the changes in human dry eye. This rat model may play an essential role for dry eye studies in the future.