| HPLC法测定冰樟桉氟轻松贴膏中醋酸氟轻松的含量 |
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| 引用本文： | 胡守莲,邱栋樑.HPLC法测定冰樟桉氟轻松贴膏中醋酸氟轻松的含量[J].海峡药学,2014(12):51-53. |
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| 作者姓名： | 胡守莲 邱栋樑 |
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| 作者单位： | 河南省信阳市食品药品检验所信阳464000 |
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| 摘 要： | 目的：建立冰樟桉氟轻松贴膏中醋酸氟轻松含量的HPLC 测定方法。方法采用反相高效液相色谱法，使用 DIONEX Acclaim 120 C18（250mm ×4．6mm，5μm）色谱柱，以甲醇－乙腈－水（45∶10∶45）作为流动相，检测波长240nm，流速1．0mL· min －1，柱温为30℃。结果醋酸氟轻松在0．05～0．5μg范围内线性较好（r＝0．9999），平均回收率为98．17％（RSD为0．67％）。结论该方法快速、简便、可靠、准确度高、重复性好，处方中其他成分对测定亦无干扰，分离效果好，可用于冰樟桉氟轻松贴膏中醋酸氟轻松含量测定。
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| 关 键 词： | 冰樟按氟轻松贴膏 醋酸氟轻松 HPLC 含量测定 |
Determination of FIuocinonide in BorreoI Camphor EucaIyptus OiI by HPLC |
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| HU Shou-Iian,Qiu Dong-Iiang.Determination of FIuocinonide in BorreoI Camphor EucaIyptus OiI by HPLC[J].Strait Pharmaceutical Journal,2014(12):51-53. |
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| Authors: | HU Shou-Iian Qiu Dong-Iiang |
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| Institution: | liang (Xinyang Institute for Food and Drug Control of Henan province; Xinyang 464000, China) |
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| Abstract: | OBJECTIVE To establish a method for content determination of Fluocinonide in Borreol Camphor Eucalyptus Oil by HPLC.METHODS The experimental condition of the RP-HPLC method was as follows：DI-ONEX Acclaim 120 C18 （250mm ×4.6mm,5μm）,with linear gradient elution using methanol and acetonitrile and water（45∶10∶45）,The detected wavelength was 240nm,The flow rate was 1.0mL· min -1,The column temperature was at 30℃.RESULTS The Fluocinolone curve was linear in the range of 0.05~0.5μg（ r=0.9999 ）.The aver-age recovery rate was 98.17%with RSD of 0.67%.CONCLUSION The method is simple ,reliable ,accurate and could be used in the determination of Fluocinonide in Borreol Camphor Eucalyptus Oil . |
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| Keywords: | Borreol Camphor Eucalyptus Oil Fluocinolone HPLC Determination |
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